We have developed soluble extracts from Neurospora crassa capable of accurately initiating the transcription of cloned Neurospora protein-encoding genes by RNA polymerase II in vitro. The genes encoding glutamate dehydrogenase (am) and histones H3 and H4 were transcribed by the extracts, and transcription was sensitive to alpha-amanitin at 1 mg/ml. The 5' heterogeneity of the in vitro initiatio...

13. KOCH, L. H. and McMEEKIN, T. L. A new direct nesslerization microkjeldahl method and a modification of the nessler-folin reagent for ammonia. Jour. Amer. Chem. Soc. 46: 2066-2068. 1924. 14. KVAMME, E. and HELLMAN, L. Isolation of pyruvic and alpha-ketoglutaric acids from blood and tissues in the presence of carbon-14 acetate. Anal. Chem. 26: 1995-1997. 1954. 15. OWENS, R. G. Metabolism of f...

The isolation and characterization of the circular mitochondrial plasmid pUG1 from the ascomycete Cryphonectria parasitica is described. The entire sequence (4182 bp) was obtained and high similarities to DNA-dependent DNA polymerases were revealed. Strikingly common features with the DNA polymerases encoded by the Neurospora intermedia plasmids Fiji and LaBelle, such as matches to the conserve...

Kinsky, Stephen C. (Washington University, St. Louis, Mo.). Effect of polyene antibiotics on protoplasts of Neurospora crassa. J. Bacteriol. 83:351-358. 1962.-The polyene antibiotics nystatin, amphotericin B, and filipin induced shrinkage of Neurospora crassa protoplasts stabilized in sucrose. At low concentrations of filipin (5 mug per ml), or higher concentrations of nystatin and amphotericin...

Although the role of carbon dioxide in metabolism has been examined in an extensive variety of animal and plant tissues (l), especially in microorganisms (2), to the authors’ knowledge the literature is devoid of any such references for Neurospora. This mold is particularly appropriate for such studies in view of the many well known mutants of diverse metabolic potentialities which might reveal...

A Spore killer trait, which exhibits genetic and cytological properties analogous to those previously found in Neurospora, exists in natural populations of the fungal plant pathogen Fusarium moniliforme. The genogeography of the polymorphism in F. moniliforme differs from the situation in Neurospora intermedia. It is more akin to the situation in N. sitophila, although more extreme with respect...

In this study, we present the synthesis of chiral fragrance aldehydes, which was tackled by a combination chemo-catalysis and multi-enzymatic in vivo cascade reaction development highly versatile high-throughput assay for enzymatic reduction carboxylic acids. We investigated biocompatible metal-catalyzed preparation ? or ? substituted cinnamic acid derivatives were fed directly into biocatalyti...

Neurospora crassa is the only molecular genetic model system for circadian rhythms research in the fungi. Its strengths as a model organism lie in its relative simplicity--compared to photosynthesizing and vertebrate organisms, it is a stripped-down version of life. It forms syncitial hyphae, propagates and reproduces, and the circadian clock is manifest in numerous processes therein. As with o...

The finding by Tatum, Banner, and Beadle (l), that the tryptophanless Neurospora mutant strain 10575 accumulates anthranilic acid, which in turn can be utilized for growth of strain 40008, has provided evidence that anthranilic acid is a biochemical precursor of tryptophan in this organism. It has been further established that indole is an intermediate in this conversion (2-5). More recent work...