background: toxoplasmosis is considered as one of the most common infectious diseases caused by the protozoan parasite toxoplasma gondii . tachyzoite is the main form of toxoplasma and continuously is maintained in cell culture or injected into the mice peritoneal cavity. this study was designed to evaluate the survival rate of rh strain of t. gondii tachyzoites in different cell free, nutrient...

The pollen of five diploid and two tetraploid guayule (Parthenium argentatum Gray) plants was cultured on nutrient media. It was observed that guayule pollen is released as tetrads of spores which will not ger­ minate on the media unless they are separated into individual pollen grains. For each plant, the results of the germination tests varied with the day and the time of day of the pollen co...

Callus induction, somatic embryogenesis and plant regeneration were initiated in selected five species of Turkish crocus using three diffrent explants (leaf, stem and corm) cultured on four different media (MS, GB5, LS and CHE). The highest frequencies of callus induction (100%) and shoot regeneration (70%, with 7.2 shoots/callus) were found in the crocus species Crocus oliveri ssp. Oliveri, us...

Aspergillus penicillioides is a true halophile, present in diverse econiches - from the hypersaline athalassohaline, and thalassohaline environments, to polyhaline systems, and in different geographical locations. Twenty seven isolates from these environments, were seen to be moderate halophiles, euryhaline in nature. They had an obligate need of a low aw and were unable to grow on a regular de...

Manganese (Mn), an essential elemental nutrient, is known to be neurotoxic at high occupational levels. We examined the transport of Mn across a monolayer of rat brain endothelial cell (RBE4) to evaluate whether an electromotive permeability mechanism is responsible for Mn transport across the blood-brain barrier (BBB). The (54)Mn(2+) apparent permeability and flux showed significant temperatur...

Bacterial cultures of bacillus species capable of producing bacitracin were isolated from local habitats (garden soil, timber yard soil and sea water) and screened for the production of bacitracin on nutrient media against test organisms viz: Micrococcus luteus,(ATCC 9341) & Staphylococcus aureus (ATCC 13565).Optimization of culture conditions and media composition for bacitracin production was...

Producing phosphorus (P) fertilizers with recycled P is desirable for efficient use of P resource. However, the current cost of P recycling facilities in Japan strongly discourages the government from adopting this practice. To expand consideration for a P recycling policy, the concept of social externality was introduced. Social issues, such as the violation of human rights in P mining in the ...

In the present investigation an attempt has been made to enhance rosmarinic acid level in plants, grown in vitro, of 2 species of Mentha in presence of 2 precursors in the nutrient media during culture. For in vitro culture establishment and shoot bud multiplication, MS basal media were used supplemented with different concentrations and combinations of different growth regulator like NAA (alph...