A new IS-anionic stationary phase was synthesized to make on-line extraction of the ochratoxin A and B from samples of beer for HPLC. The propyltriethylammonium chloride stationary phase was characterized affecting it's elementary determination and RI specter, respectively. Evaluation of the IS-anionic column for the extraction and quantification of OTA and OTB in beer has shown that the column...

Samples of blood, kidney and liver were randomly selected from slaughtered pigs (n=90) and analyzed for ochratoxin A by HPLC. In addition, in order to obtain information on the occurrence of nephropathy, histological examinations were carried out. Of the 90 liver samples, 26.6% contained OTA in the range of 0.22-14.5 ng/g. The incidence of OTA in serum and kidney were very similar (31%, 33.3%),...

Ochratoxin A (OTA) is one of the most important mycotoxins, and contaminates several agricultural products, particularly cereals, grapes, maize, barley, spices and coffee. The aim of this project was to reduce the levels of OTA by supplementing the artificially contaminated solutions with seven strains of actinobacteria (AT10, AT8, SN7, MS1, ML5, G10 and PT1) in order to evaluate their capacity...

Soluble coffee and roasted coffee beans were purchased on the local market and analysed for OTA using aminopropylsolid phase extraction column clean-up and quantitation with reversed phase HPLC. Preliminary results suggest that the levels of OT A in coffee on the South African market are slightly higher than on the European market. The levels varied between undetectable to 40 ng OT A per gram c...

Ochratoxin A (OTA) is a very dangerous mycotoxin, the presence of which is often reported in different foods, as well as in beverages such as grapes, grape juices and wines. Detoxifying these products is therefore of prime importance in protecting consumer health, and biological approaches have been the most promising methods. In this report, 40 isolates representing the black apergilli species...

This experiment was conducted to evaluate the effects of ochratoxin A (OTA) on the function and histology of the liver, kidney and bursa of broiler chickens fed OTA contaminated rations. Two hundred forty one-day-old male Ross broiler chicks were used, they were randomly divided into 3 dietary experimental groups of 80 birds and given rations containing 0 (control), 400 or 800 μg OTA/Kg feed. T...

Abbreviations: OTA: Ochratoxin-A; hr: Hour; ppm: Parts-Per Million; b.wt.: Body Weight; RBC: Red Blood Cell; Hb: Hemoglobin; PCV: Packed Cell Volume; TLC: Total Leukocyte Count; DLC: Differential Leukocyte Count; MCV: Mean Corpuscular Volume; MCH: Mean Corpuscular Hemoglobin; MCHC: Mean Corpuscular Hemoglobin Concentration; BUN: Blood Urea Nitrogen; ALP: Alkaline Phosphatase; ALT: Alanine Trans...

Ochratoxin A (OTA) is a mycotoxin produced by different Aspergillus and Penicillium species. Since its mechanism of action is not fully understood yet, it is important to gain further insight into different interactions of OTA at the molecular level. OTA is found worldwide in many foods and drinks. Moreover, it can also be detected in human and animal tissues and body fluids, as well. Therefore...

The aim of this study was to monitor the mycobiota of wine grapes, occurrence of ochratoxigenic microfungi in wine grapes and occurrence of ochratoxin A and Alternaria mycotoxins in fresh grape juice, must and wine from domestic crops in the year 2004. Thirteen samples of wine grapes (white /nine samples/ and red /four samples/) were collected during harvesting in the Znojmo wine region, SE Mor...

Zearalenone (ZEN) and ochratoxin A (OTA) are secondary toxic metabolites widely present in grains and grain products. In this study, the effects of electron beam irradiation (EBI) on ZEN and OTA in corn and the quality of irradiated corn were investigated. Results indicated that EBI significantly affected ZEN and OTA. The degradation rates of ZEN and OTA at 10 kGy in solution were 65.6% and 75....