The ideal tumor antigen is one expressed selectively by the tumor, present in all cancer patients, essential for tumor survival and nonetheless able to induce both humoral and cellular immune response. The personalized idiotype (Id) of the surface immunoglobulin is a tumor specific antigen in that it is expressed on clonal B-cell tumors, mediates B-cell survival, and induces tumor specific immu...

Transcriptional gene silencing has been reported with siRNA targeting the promoter region of genes. We tested several siRNAs directed against the human VEGF promoter. Of these, siVFp(-992) exhibited > or =50% suppression of VEGF production in two human cell lines. To determine the specificity of this siRNA-mediated suppression, plasmids were prepared to express a luciferase reporter under the c...

objective: the greatest challenge in cancer gene therapy is to achieve the high specificity and efficiency in targeting of cancer cells. because the goal of cancer gene therapy is to eradicate cancer cells, many therapeutic genes could be detrimental if unintentionally expressed in normal cells. using promoter of the genes which are expressed specifically in cancer cells or have much more expre...

multiple gene silencing is being required to target and tangle metabolic pathways in eukaryotes and researchers have to develop a subtle method for construction of rna interference (rnai) cassettes. although, several vectors have been developed due to different screening and cloning strategies but still some potential limitations remain to be dissolved. here, we worked out a simple cloning stra...

objective(s): p-glycoprotein (p-gp) is an efflux protein, the overexpression of which has been associated with multidrug resistance in various cancers. although sirna delivery to reverse p-gp expression may be promising for sensitizing of tumor cells to cytotoxic drugs, the therapeutic use of sirna requires effective carriers that can deliver sirna intracellularly with minimal toxicity on targe...

This research has concentrated on the physical and socio-economic impacts of vegetation-based management scenarios targeting on flooding and soil erosion issues in the Ramian watersheds. The Ramian watershed with an approximate area of 24000 ha is located in Golestan, Iran. For each sub-watershed, four biological actions (activities) and 16 management scenarios have been considered. Physical im...

Triage in emergency response refers to determining the priority of victims based on their need for treatment and medical intervention. Today, triage is performed by the use of paper-based triage tags. Communication about patients' status is mainly carried out over radio or through handwritten notes. This practice makes it challenging for emergency personnel to keep an overview of the number, lo...

While many aspects of tutoring have been identified and studied, off topic conversation has been largely ignored. In this paper, off topic conversation during 50 hours of one-to-one expert tutoring sessions was analyzed. Two distinct methodologies (Dialogue Move occurrence and LIWC analysis) were used to determine the anatomy of off topic conversation. Both analyses revealed that the expected s...

Since highway improvement project selection requires screening thousands of road segments with respect to crashes for further analysis and final project selection, we provide a two-step project selection methodology and describe an application case to demonstrate its advantages. In the first step of the proposed methodology, we will use odds against observing a given crash count, injury count, ...