rate of in vitro maturation of oocytes is one of the challenges of assisted reproductive techniques. in this study we investigated the effects of supplementation of cysteamine on the rate of in vitro maturation of oocytes in two different media. germinal vesicle oocytes were collected from mouse ovary and cultured in two media (tcm199 and meme) with 0, 50, 100, 200, 500 µm/ml cysteamine. number...

the purpose of this study was to evaluate the use of glass capillary micropipette (gcm) as a vessel forvitrification of bovine oocytes. cumulus-oocyte complexes (cocs) were obtained from slaughter-house andwashed 5 to 6 times in the washing medium (tcm-199 + 20% fbs) and randomly assigned to treatment andcontrol group. in the first step of vitrification, cocs were exposed to first vitrification...

pelvic inflammatory disease (pid) is a rare complication of transvaginal oocyte retrieval. it may result in failure of assisted reproductive techniques (art). during a 7 years period, 5958 transvaginal ultrasound-guided oocyte retrievals resulted in 10 cases of acute pid. eight out of 10 patients were diagnosed infertile because of endometriosis. two patients had mild ovarian, 3 had stage iii, ...

objective: currently, there is no agreement on the optimal urinary derived human chorionic gonadotropin (u-hcg) dose requirement for initiating final oocyte maturation prior to oocyte collection in in vitro fertilization (ivf), but doses that range from 2500- 15000 iu have been used. we intended to determine whether low dose u-hcg was effective for oocyte maturation in ivf/intracytoplasmic sper...

Cell-to-cell communication was characterized in cumulus-oocyte complexes from rat ovarian follicles before and after ovulation. Numerous, small gap junctional contacts were present between cumulus cells and oocytes before ovulation. The gap junction are formed on the oocyte surface by cumulus cell processes that transverse the zona pellucida and contact the oolemma. The entire cumulus mass was ...

Background: In vitro maturation (IVM) is a promising treatment option for certain infertile women. Nowadays, with the aid of the Polscope, it has become possible to evaluate zona pellucida (ZP) and spindle as parameters of oocyte quality. The goal was to investigate the relationship between the presence of the meiotic spindle and ZP birefringence with morphology of the in vivo and in vitro matu...

In this study, the authors examined late atretic follicles in ovaries of Atlantic bluefin tuna, Thunnus thynnus (Linnæus 1758), at different times year using transmitted light and epifluorescence microscopy. Atresia (degeneration resorption developing ovarian follicles) is a natural process involved fecundity downregulation teleosts substantially enhanced stressful conditions. Early (? ?) stage...

CAPSULE Mechanisms that control the survival of oocytes and, by extension, the duration of ovarian function have been identified. However, it is still not clear whether oocyte "quality" is related to survival, nor is the role of the granulosa cells of follicles in follicle survival entirely understood. Here, we consider oocyte-intrinsic and oocyte-extrinsic mechanisms of oocyte loss and argue t...

Polarity of the Drosophila oocyte is essential for correct development of the egg and future embryo. The Par proteins Par-6, aPKC and Bazooka are needed to maintain oocyte polarity and localize to specific domains early in oocyte development. To date, no upstream regulator or mechanism for localization of the Par proteins in the oocyte has been identified. We have analyzed the role of the small...