A new porcine parvovirus (PPV) was identified from pig lung tissues in the United States. It is most closely related to the recently identified PPV4 with overall genomic identities of 64.1 to 67.3%. Unlike PPV4, this virus lacks the additional open reading frame 3 (ORF3) and has a much longer ORF2. The name PPV5 is provisionally proposed.

The gene region coding for lithotrophic sulfur oxidation of Paracoccus pantotrophus GB17 is located on a 13-kb insert of plasmid pEG12. Upstream of the previously described six open reading frames (ORFs) soxABCDEF with a partial sequence of soxA and soxF (C. Wodara, F. Bardischewsky, and C. G. Friedrich, J. Bacteriol. 179:5014-5023, 1997), 4,350 bp were sequenced. The sequence completed soxA, a...

The genome of pineapple mealybug wilt-associated closterovirus-2 (PMWaV-2) was cloned from double-stranded RNA isolated from diseased pineapple and its sequence determined. The 3'-terminal 14861 nt of the single-stranded RNA genome contains ten open reading frames (ORFs) which, from 5' to 3', potentially encode a >204 kDa polyprotein containing papain-like protease, methyltransferase and helica...

TT virus (TTV) was recovered from the sera of tupaias (Tupaia belangeri chinensis) by PCR using primers derived from the noncoding region of the human TTV genome, and its entire genomic sequence was determined. One tupaia TTV isolate (Tbc-TTV14) consisted of only 2199 nucleotides (nt) and had three open reading frames (ORFs), spanning 1506 nt (ORF1), 177 nt (ORF2) and 642 nt (ORF3), which were ...

A 7.0-kb DNA fragment that conferred redbrown pigment production on Streptomyces griseus was shotgun-cloned with a multicopy vector pIJ486 from this microorganism. By restriction endonuclease mapping and subcloning, a 1.5-kb fragment which is essential for the production of redbrown pigment was determined. The nucleotide sequence of this region revealed the presence of two open reading frames, ...

Due to the lack of national analogies a prototype enzyme-linked immunosorbent assay (ELISA) test system was developed for qualitative determination antibodies class G hepatitis E virus (HEV) based on using antigenic polypeptides ORF2 and ORF3 3rd genotype (FSBSI «I.I. Mechnikov Research Institute Vaccines Serums», Russia). The results experimental ELISA conducted in parallel with reference «DS-...