Tissue engineering-based bone grafts are emerging as a viable alternative treatment modality to repair and regenerate tissues damaged as a result of disease or injury. The choice of the biomaterial component is a critical determinant of the success of the graft or scaffold; essentially, it must induce and allow native tissue integration, and most importantly mimic the hierarchical structure of ...

Three-dimensional (3D) cell constructs are expected to provide osteoinductive materials to develop cell-based therapies for bone regeneration. The proliferation and spontaneous aggregation capability of induced pluripotent stem cells (iPSCs) thus prompted us to fabricate a scaffold-free iPSC construct as a transplantation vehicle. Embryoid bodies of mouse gingival fibroblast-derived iPSCs (GF-i...

Developing a successful bone tissue engineering strategy entails translation of experimental findings to clinical needs. A major leap forward toward this goal is developing a quantitative tool to predict spatial and temporal bone formation in scaffold. We hypothesized that bone formation in scaffold follows diffusion phenomenon. Subsequently, we developed an analytical formulation for bone form...

Bone marrow stromal cells (BMSCs) are considered a promising tool for bone bioengineering. However, the mechanisms controlling osteoblastic commitment are still unclear. Osteogenic differentiation of BMSCs requires the activation of β-catenin signaling, classically known to be regulated by the canonical Wnt pathway. However, BMSCs treatment with canonical Wnts in vitro does not always result in...

INTRODUCTION: One in two women and one in four men over the age of 50 will have an osteoporosisor low bone mass-related fracture in their lifetime. Delayedor non-union occurs in 10–15% of patients resulting from various risk factors including fracture location, poor mechanical stability, and aging. Traditional treatment strategies include internal fixation and autologous bone graft, but challen...

Synthetic and natural polymer association is a promising tool in tissue engineering. The aim of this study was to compare five methodologies for producing hybrid scaffolds for cell culture using poly-l-lactide (PLLA) and collagen: functionalization of PLLA electrospun by (1) dialkylamine and collagen immobilization with glutaraldehyde and by (2) hydrolysis and collagen immobilization with carbo...

Stem cells biology is one of the most frequent topic of physiological research of today. Spinal fusion represents common bone biology challenge. It is the indicator of osteoinduction and new bone formation on ectopic model. The purpose of this study was to establish a simple model of spinal fusion based on a rat model including verification of the possible use of titanium microplates with hydro...

Injectable bone implants have been widely used in bone tissue repairs including the treatment of comminuted bone fractures (CBF). However, most injectable bone implants are not suitable for the treatment of CBF due to their weak tissue adhesion strengths and minimal osteoinduction. Citrate has been recently reported to promote bone formation through enhanced bioceramic integration and osteoindu...

Background: Tooth extraction is surgical treatment with clinical manifestation alveolar bone resorption of 11-63% which potentially fails dental implant treatment. Socket preservation regeneration to minimize morphological changes in post-extraction using graft. Anti-inflammatory biomaterial needed modulate inflammatory cascade the lesion. Anthocyanin ternatin a flavonoid derived from butterfly...

With the pursuit of new cancer therapies and more effective treatment to diseases in last decades, nanotechnology has been an important ally for healthcare professionals patients critical clinical conditions. Nanomaterials offer alternative way deliver toxic chemotherapeutic drugs specific biological tissues, cells or microbial beings, resulting avoidance strong side effects resilience drugs. A...