In this study, five carp pituitary glands were collected and dispersed enzymatically and mechanically.Then, the cells were cultivated as monolayer in MEM (minimum essential medium Eagle). The culture mediawere collected after 72 h and frozen at -20°C. Carp ovarian follicles also were separated mechanically andincubated in BSS (basic salt solution) Cortland medium in 24-well microplates for 48 h...

Background: In vitro culture of ovarian follicles may preserve fertility in women with premature ovarian failure due to cancer .It seems that creation a condition that could maintain cellular communications and supports growth of follicles to produce mature oocytes appear to be essential. Nitric oxide (NO) has been recently shown to act with a dual action in mouse oocyte meiotic maturation depe...

BACKGROUND Endothelium dysfunction is believed to play a role in the development of cardiovascular disease. The aim of the present study was to evaluate the suitability of organ culture as a model for endothelium dysfunction. METHODS The isometric tension was recorded in isolated segments of the rat mesenteric artery branch, before and after organ culture for 20 h. Vasodilatation was expresse...

in this study, five carp pituitary glands were collected and dispersed enzymatically and mechanically.then, the cells were cultivated as monolayer in mem (minimum essential medium eagle). the culture mediawere collected after 72 h and frozen at -20°c. carp ovarian follicles also were separated mechanically andincubated in bss (basic salt solution) cortland medium in 24-well microplates for 48 h...

The purpose of the study was to test the possible role of the androgen receptor (AR) agonist (testosterone; T), an AR antagonist (2-hydroxyflutamide; 2-Hf) or combination of both (T+2-Hf) on progesterone receptor (PGR) expression in cultured porcine granulosa cells (GCs) or whole follicles. GCs isolated from mature pig follicles (6-8mm in diameter) were cultured for 48 h. Experimental cultures ...