Chitin is a polysaccharide component of the inner cell wall fungi that has been used to estimate fungal invasion in plant products. However, its detection major pathogenic species not investigated. The present study aimed determine chitin contents order evaluate diagnostic potential as biomarker for infections. High performance liquid chromatography (HPLC) was measure content. Pure acid hydroly...

introduction candidiasis as an opportunistic infection is created by some species of candida. here the role of candida albicans is more noticeable than other species. candida can cause an extended spectrum of symptoms. due to the increasing consumption of immunosuppressants including corticosteroids and diseases like diabetes, candidiasis has drawn more attention than before. methods considerin...

introduction: curcumin is the active ingredient in the traditional herbal remedy and dietary spice turmeric (curcuma longa). curcumin has a surprisingly wide range of beneficial properties, including anti-inflammatory, antioxidant, chemopreventive and chemotherapeutic activity. on basis of recent studies; it has antifungal and antibacterial effects. the aim of this study was in-vitro evaluation...

A heterologous fusion between mouse myeloma cells and rat lymphocytes resulted in the isolation of a rat immunoglobulin M monoclonal antibody with both agglutinating and precipitating activity. Indirect immunofluorescence and direct agglutination tests showed that the corresponding antigen was present in the cell wall of the three Candida species considered to be the most pathogenic, C. albican...

Cell wall beta-1,2-oligomannosides are involved in Candida albicans binding to macrophages and in their stimulation to produce cytokines. The nature of signaling events occurring during initial interaction of macrophage J774 cell line and C. albicans, together with the nature of molecules containing beta-1,2-oligomannosides released by the yeasts, was examined. Cocultivation led to a herbimycin...

Sphingolipids are involved in several cellular functions, including maintenance of cell wall integrity. To gain insight into the role of individual genes of sphingolipid biosynthetic pathway, we have screened Saccharomyces cerevisiae strains deleted in these genes for sensitivity to cell wall perturbing agents calcofluor white and congo red. Only deletants of FEN1 and SUR4 genes were found to b...

results the minimum inhibitory concentrations (mics) of ginger extract for evaluated strains were 40, 40, 20, 20, 20, 20, 10, and 5 mg/ml for pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, klebsiella pneumoniae, bacillus cereus, acinetobacter baumannii, c. albicans, and c. krusei, respectively. ginger extract successfully inhibited biofilm formation by a. baumannii, b. cereus,...

The cell wall integrity pathway (CWI) plays an important role in the biogenesis of the cell wall in Candida albicans and other fungi. In the present work, the C. albicans MKK2 gene that encodes the putative MAPKK of this pathway was deleted in different backgrounds and the phenotypes of the resultant mutants were characterised. We show here that Mkk2 mediates the phosphorylation of the Mkc1 MAP...

K101 Nail Solution (trademarks Emtrix(®), Nalox(™), Naloc(™)) is a combination of propylene glycol, urea and lactic acid in a topical formulation for the treatment of nails affected by onychomycosis. The aim of this study was to investigate the Minimal Cidal Concentration (MCC) of K101 Nail Solution against Trichophyton rubrum and Candida albicans as well as the effect of K101 Nail Solution on ...

A reliable method to determine cell wall polysaccharides composition in yeast is presented, which combines acid and enzymatic hydrolysis. Sulphuric acid treatment is used to determine mannans, whereas specific hydrolytic enzymes are employed in a two sequential steps to quantify chitin and the proportion of β-(1,3) and β-(1,6)-glucan in the total β-glucan of the cell wall. In the first step, ch...