Background Actinomycetes may account for 10 to 30% of the total soil rhizosphere microorganisms. The attention given to the actinomycetes in biotechnological applications is a result of their metabolic versatility that is accompanied by the production of primary and secondary metabolites of economic importance, which are a promising source of products (e.g., antibiotics, enzyme inhibitors, anti...

Sixteen actinomycetes capable of utilizing dibenzofuran as a sole source of carbon and energy were isolated, including Rhodococcus, Microbacterium, and Terrabacter genera. Heretofore, no dibenzofuran-utilizing strain belonging to the genus Microbacterium has been reported. Five extradiol dioxygenase genes (dfdB, and edil to 4) of the strain Rhodococcus sp. YK2 were cloned and analyzed. The nucl...

Mangrove-derived actinomycetes are promising sources of bioactive natural products. In this study, using homologous screening of the biosynthetic genes and anti-microorganism/tumor assaying, 163 strains of actinomycetes isolated from mangrove sediments were investigated for their potential to produce halogenated metabolites. The FADH2-dependent halogenase genes, identified in PCR-screening, wer...

The enzyme Asparaginase, known as L-asparagine amidohydrolase, has anti-tumor properties and is widely used a chemotherapeutic treatment for acute lymphoblastic leukemia. Many different organisms, including plants terrestrial microbes such bacteria, fungi, algae actinomycetes, have produced this enzyme. In study, 34 isolates of the actinomycetes were collected from soil water governorates in Eg...

Two distinct Phytophthora taxa were found to be associated with brown rot of pomelo (Citrus grandis), a new disease of this ancestral Citrus species, in the Vinh Long province, Mekong River Delta area, southern Vietnam. On the basis of morphological characters and using the ITS1-5.8S-ITS2 region of the rDNA and the cytochrome oxidase subunit 1 (COI) as barcode genes, one of the two taxa was pro...

Actinomycetes are a diverse group of medically, industrially and ecologically important bacteria, studied as much for the diseases they cause as for the cures they hold. Along with also as a source of industrial important product which are helpful for mankind. However, many of the rare genera of actinomycetes have been neither explored nor manipulated for their biotechnological and industrial p...

Secondary Metabolites of Soil Actinomycetes, UTMC 676 and 919, Induces Apoptosis in Human Non‑Small-Cell Lung Cancer Cell Line

Screening of microorganisms for the production of novel antibiotics has been intensivelypursued for many years by scientists. Actinomycetes have the capability to synthesize manydifferent antibiotics. A total of 69 actinomycete isolates were recovered from soil samplescollected from Alborz Province. Selected colonies (rough, chalky) of actinomycetes werepurified. All screened isolates were iden...

Antibiotic drug discovery is an indispensable process to combat aggressive ability of pathogenic microorganisms and emerging infectious diseases against health and well-being of people throughout the world. According to the updated and expanded data on natural products presented by Newman and Cragg (2012), the utility of natural products as sources of novel structures is still alive and the ant...

Agar media made with 0.4% colloidal chitin plus mineral salts and adjusted to pH 8.0 was superior to four other commonly used media for the isolation and enumeration of actinomycetes from water samples. More actinomycetes developed on chitin agar, and the development of bacteria and fungi was suppressed. Frozen and vacuum-dried chitin from aqueous colloidal suspensions was finely divided and ga...