The pathways by which large RNAs adopt tertiary structure are just beginning to be explored, and new methods that reveal RNA folding are highly desirable. Here we report an assay for RNA tertiary folding in which the fluorescence of a covalently incorporated chromophore is monitored. Folding of the 160-nucleotide Tetrahymena group I intron P4-P6 domain was used as a test system. Guided by the P...

INTRODUCTION The use of acupressure is growing. Several studies have applied pressure to the P6 to reduce postoperative nausea, vomiting, and pain but have reported conflicting results. This study aimed to investigate the effects of pressure to the P6 point on pain, nausea and vomiting after appendectomy. METHODS A single-blind, randomized controlled clinical trial was conducted on 88 patient...

Human immunodeficiency virus type 1 expresses structural proteins and replicative enzymes within gag and gag-pol precursor polyproteins. Specific proteolytic processing of the precursors by the viral proteinase is essential for maturation of infectious viral particles. We have studied the activity of proteinase in its immature form, as part of a gag-pol fusion protein, in an in vitro expression...

In a strictly controlled clinical situation, (postoperative sickness) where variables were reduced to a minimum, it was possible to demonstrate an effective prophylactic antiemetic action of P6 (Neiguan) acupuncture. Manual and electrical invasive (needling) stimulation of this point were equally effective. Non-invasive stimulation (transcutaneous electrical or pressure) was effective in the ea...

We infected a transgenic Arabidopsis line (GxA), containing an amplicon-silenced 35S : : GFP transgene, with cauliflower mosaic virus (CaMV), a plant pararetrovirus with a DNA genome. Systemically infected leaves showed strong GFP fluorescence and amplicon transcripts were detectable in Northern blots, indicating that silencing of GFP had been suppressed during CaMV-infection. Transgenic Arabid...

BACKGROUND Postoperative nausea and vomiting (PONV) are common complications following surgery and anaesthesia. Drugs to prevent PONV are only partially effective. An alternative approach is to stimulate the P6 acupoint on the wrist. This is an update of a Cochrane review first published in 2004. OBJECTIVES To determine the efficacy and safety of P6 acupoint stimulation in preventing PONV. ...

In this paper, we count cycles in a generalized Kautz digraph GK (n; d). Let n = pd such that d| ,p. Also let gl = gcd(d − (−1)l; n). We show that if one of the following conditions holds: • p6 √ d7 d+1 and k6 logd n+ 1, • √ d7 d+1 ¡p¡d (d+ 1) and k6 logd ( n 3 √ p2(d+1) ) + 10 3 , • d(d+ 1)¡p and k6 logd ( n d+1 ) then the number of cycles of length k in GK (n; d) is given by 1 k ∑ l | k ( k l...

This study was conducted during the period from 2015 to 2017 resistance of powdery mildew in eight melon inbred lines and their hybrids order reduce losses quality yield.  Eight different genetically were selfed for six generations ensure homozygosity. They have been self-pollinated intercrossed a half diallel mating design obtain 28 2015. Thirty genotypes (8 Lines produced hybrids) evaluated r...

The Maximum Stable Set (MS) problem is a well known NP-hard problem. However different graph classes for which MS can be efficiently solved have been detected and the augmenting graph technique seems to be a fruitful tool to this aim. In this paper we apply a recent characterization of minimal augmenting graphs [22] to prove that MS can be solved for (P6,K2,3)-free graphs in polynomial time, ex...