The enzyme complex sucrase l isomaltase is an intrinsic dimeric glycoprotein with a molecular weight of approximately 220,000. It is anchored in the intestinal brush border membrane by a hydrophobic segment amounting to less than 10% of the total protein mass which is located near the NHz-terminal end of the isomaltase subunit. This conclusion is based on studies of (i) the mechanism of solubil...

OBJECTIVES This study sought to create a model for testing topical treatment of jellyfish stings. It sought to determine which treatments 1) stimulate/inhibit nematocyst discharge; 2) decrease pain; and 3) decrease skin inflammation; it also sought to discover whether there is a clinical correlation between stimulated nematocyst discharge observed in vitro to the pain and erythema experienced b...

The aim of the paper was to evaluate mutual relations in the system of alveolar macrophage (AM) - type II pneumocyte (PII) - interstitium of alveolar septa, in the course of experimental lung emphysema in rats subjected to BCG vaccine effect. Administration of BCG vaccine resulted in the cumulation of AM within pulmonary alveoli. These cells exhibited morphological features of increased activit...

We have determined the kinetic parameters for the hydrolysis by papain, cathepsin B and cathepsin L of internally quenched fluorescent peptides derived from the lead peptides Abz-AAFRSAQ-EDDnp [in which Abz and EDDnp stand for o-aminobenzoic acid and N-(2,4-dinitrophenyl)ethylenediamine respectively], to map the specificity of S(4) and S(3) subsites, and Abz-AFRSAAQ-EDDnp, to identify the speci...

The objective of this study was to determine the antimicrobial activity of proteolytic, meat-tenderizing enzymes (papain and bromelain) against E. coli and L. monocytogenes at three different temperatures (5, 25 and 35°C). Two overnight cultures of E. coli JM109 and L. monocytogenes were separately suspended in 1% peptone water and exposed to a proteolytic enzyme (papain or bromelain) at three ...

We present an analysis of the electrostatic properties in the catalytic site of papain (EC 3.4.22.2), an archetype enzyme of the C1 cysteine proteinase family, and we investigate their possible role in the formation, stabilization and regulation of the Cys25((-))...His159((+)) catalytic ion pair. The electrostatic properties were computed using a reassociation method based in multicentered mult...

This study investigated the influence of a papain-based gel (Papacárie) for chemo-mechanical caries removal on bond strength to dentin. Human molars were assigned to the following groups: Group 1: sound teeth were flattened to expose dentin; Group 2: after flattening of surfaces, the papain-based gel was applied on the sound dentin; Group 3: overlying enamel from carious teeth was removed and m...

7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD chloride) and 7-(2'-hydroxyethylthio)-NBD (obtained from NBD chloride and mercaptoethanol) undergo a reversible spectral change in alkaline solution that depends respectively on a single apparent pK(a) 9.76 (at 25 degrees C) and 8.81 (at 32 degrees C). In acid solution however no spectral change was observed. NBD chloride reacts slowly with papain at...

Our model o f chymopapain M reveals differences from papain in the S,-subsite (see [ 18, 1 Y ] for schematic diagrams), where P68 and W6Y in papain are respectively E68 and S6Y in chymopapain M. These differences may affect substrate binding. It is interesting also that Y61. Y67, E62 and HXI. suggcstcd as possible candidates for modulators o f papain rcactivity and activity [ 1 XI, are conserve...

was first shown by Henrique and Andersen (1913). Elman and Lischer (1943) administered large amounts of amino-acids and peptides intravenously to men without any ill effects. They were able thus to maintain nitrogen equilibrium in patients who could not take food by mouth. Bassett et al. (1944) report a study of a patient with gastro-jejuno-colic fistula, who was given solutions of amino-acids ...