Yeast mitochondrial DNA codes for eight major polypeptides. Translation of he mitochondrially encoded polypeptides in strains with mutated mitochondrial release factor, mRF1, was found to result in the synthesis of a novel protein, V2. Different mrf1 alleles were associated with different efficiency of V2p synthesis. Translation of V2p was enhanced by paromomycin. Comparative analysis of peptid...

Parenteral pentavalent antimonials remain the standard therapy for cutaneous leishmaniasis. More effective and patient-compliant topical treatments are an important alternative treatment for the localized self-limiting forms of this disease. Two paromomycin ointments are commercially available but their use is limited by either toxicity or lack of efficacy. Other topical formulations have been ...

Sir: The accomplishment of an efficient method for obtaining the previously unknown 1,3,2"',6"'tetra-N-acetylparomomycin (1) and the subsequent preparation of the pseudotrisaccharide 5-0[3-0(2,6-diami no-2,6-dideoxy-f-L-idopyranosyl)-/3-L-ribo-furanosyl]-2-deoxystreptamine(5), as described in the preceding papery, by nitrous acid deamination, suggested a route for providing a useful intermediat...