A 10% rat liver homogenate in 0.25 M sucrose-10-3 M ethylenediaminetetraacetate was fractionated into mitochondria, microsomes, and a supematant fraction. Liver mitochondria derived from rats injected with leucine-3H and with phosphate-32P or with glycerolJ4C were incubated with nonlabeled microsomes in the presence of supernatant or of sucrose-EDTA. Labeled microsomes were similarly exposed to...

Introduction: The production of anti-Cryptosporidium polyclonal antibodies and its use in direct immunofluorescence assays to determine the presence of Cryptosporidium in water are described in the present work. Methods: Two rabbits were immunized with soluble and particulate antigens from purified Cryptosporidium oocysts. The sera produced were prepared for immunoglobulin G extraction, which w...

135 ABSTRACT: Heavy metals and dyes are major contributors in contamination of water streams. These contaminants enter into our ecosystem, thus posing a significant threat to public health, ecological equilibrium and environment. Thus a combined discharge of these contaminants results in water pollution with high chemical oxygen demand, biological oxygen demand, color, particulate matter, suspe...

In aquatic environments, the term humic substances (HS) encompasses terrestrial and autochthonous refractory organic matter. HS are one of main fractions natural matter important chelators trace elements. environmental studies, determination is often restricted to dissolved fraction, content in suspended particles remains unknown. this work, we present composition dynamics along mixing gradient...

Seven or eight penicillin-binding proteins (PBPs) were detected in Bacillus subtilis membranes. By introducing covalent affinity chromatography employing cephalosporins as ligands, milligram amounts of three high molecular weight PBPs (PBP 1 ab, Mr = 120,000; PBP 2b, Mr = 94,000; and PBP 4, Mr = 78,000) were obtained without any contamination of the major PBP 5, the D-alanine carboxypeptidase. ...

The inhibition of colony-forming efficiency of cultured human embryonic intestinederived epithelial (I-407) cells was utilized in order to assay the toxic potential of six coded samples of particulate matter provided by the United States Environmental Protection Agency (EPA). The results of the assay indicated that the most toxic of the EPA samples tested “blindly” was amosite which was equal i...

We review the evolution, state of the art and future lines of research on the sources, transport pathways, and sinks of particulate trace elements in urban terrestrial environments to include the atmosphere, soils, and street and indoor dusts. Such studies reveal reductions in the emissions of some elements of historical concern such as Pb, with interest consequently focusing on other toxic tra...

Direct emission of cadmium into air and water in Japan has been controlled on the basis of the Emission and Effluent Standards. Environmental quality in these media has been improved, but delayed effects of these emissions in the past are now manifest by contamination of agricultural acreage in various parts of this country. Special land improvement projects are therefore required, and cost all...

The use of strains of Salmonella enterica serovar Typhimurium with different metabolic capacities can indicate the class or classes of compounds present in an environmental sample and enable the diagnosis of the mutagenic activity of these pollutants adsorbed on particulate matter (PM) in the air. In the present study, the sensitivity of Salmonella strains TA98NR, TA98/1,8-DNP₆, YG1021, and YG1...

The use of seabirds as sentinels of the condition of aquatic ecosystems has been well-established. Large environmental perturbations to aquatic food webs (e.g., chemical contamination, overfishing, particulate pollution) have all been detected or monitored by tracking seabirds at colonies. However, seabirds may elicit more subtle, sublethal responses that can also be used to track ecosystem hea...