This study investigated the ability to reprogramming human fibroblast cells into hepatocyte-like by overexpressing HNF4a gene. The isolated and primary cultured from foreskin donated healthy patients. gene overexpressed through Doxycilin activatedthe vectors Tet-on-eGFP-HNF4a. after process were evaluated observing morphological changes, immunofluorescence staining assess expression of proteins...

Previous studies have shown that injured dorsal column sensory axons extend across a spinal cord lesion site if axons are guided by a gradient of neurotrophin-3 (NT-3) rostral to the lesion. Here we examined whether continuous NT-3 delivery is necessary to sustain regenerated axons in the injured spinal cord. Using tetracycline-regulated (tet-off) lentiviral gene delivery, NT-3 expression was t...

OBJECTIVES Hypoxia induces cellular oxidative stress that is associated with neurodegenerative diseases. Here, the protective effects of ferulic acid (FA) on hypoxia-induced neurotoxicity in PC12 cells were evaluated. MATERIALS AND METHODS We investigated the effect of FA on PC12 cells subjected to hypoxia stress, in vitro. RESULTS FA increased cell viability, prevented membrane damage (LDH...

As bstract. Normal volunteers received either initial or booster immunization with tetanus toxoid. Bone marrow and peripheral blood mononuclear cells were obtained for up to 28 d after immunization and were analyzed for synthesis of total Ig and specific antibodies to tetanus toxoid. Cells were cultured in vitro for 3 or 7 d with or without pokeweed mitogen (PWM). Synthesis of IgG and IgM antib...

Vortex Induced Vibrations (VIV) forms the basis of the hydrokinetic energy converter. With the increasing energy demand, there is a need for a significant shift to using clean and renewable sources of energy. VIV based hydrokinetic energy converter aims to harness aquatic energy from slow moving water currents. For modeling, a single cylinder system with single degree-of-freedom is considered. ...

The PC12 line of nerve growth factor (NGF)-responsive rat pheochromocytoma cells was used as a model system to determine whether properties of microtubules change during neurite growth and maturation. In the absence of NGF, PC12 cells lack processes. After several days with NGF, PC12 cells begin extending neurites and, by 2-3 wk with NGF, PC12 cells have long (approximately 1 mm), highly branch...

Antibodies raised in rabbits and in guinea pigs against nerve growth factor-treated PC12 cells were absorbed exhaustively with three non-neuronal cell lines. In immunofluorescent staining experiments, these absorbed sera, designated anti-PC12, labeled specifically several different types of cultured neurons, including cerebral, cerebellar, spinal, dorsal root, and superior cervical neurons. Non...

UNLABELLED The adequacy of cerebral tissue oxygenation (PtO(2)) is a central therapeutic end point in critically ill and anesthetized patients. Clinically, PtO(2) is currently measured indirectly, based on measurements of cerebrovascular oxygenation using near infrared spectroscopy and experimentally, using positron emission tomographic scanning. Recent developments in electron paramagnetic res...

Objective(s): The role of aluminum (Al) in the pathogenesis of neurodegenerative diseases has been implicated in several studies. However, the exact mechanisms of cytotoxic effects of Al have not been elucidated yet. The aim of this study was to investigate the effect of L-type calcium channel antagonist, nimodipine (NM), and lithium chloride (LiCl) on Al-induced toxicity in PC12 cells. Materia...