This study developed a new multiplex polymerase chain reaction (m-PCR) for rapidly detecting clinically essential strains of V. parahaemolyticus. enables the detection total and potentially virulent strains. The m-PCR was by targeting species-specific transcriptional regulator toxR gene, sequences an outer membrane protein hypothetical encoded omp htp, respectively. htp were discovered original...

Objective : The study aimed to evaluate molecular and immunological methods propose a workflow using them for tuberculosis (TB) diagnosis routine. Methods A cross-sectional retrospective was performed, including 121 liquid cultures from TB laboratory located in the extreme south of Brazil. All were positive Mycobacterium complex (MTBC) by in-house Polymerase Chain Reaction (PCR) DNA extracted C...

In this study, two PCR-based methods (MSP-PCR and PCR-MP) were compared for their abilities to identify intraspecies variations of 23 isolates of Trichophyton rubrum, 78 isolates of Trichophyton interdigitale and 22 isolates of Microsporum canis, obtained mainly from patients in Lódź city. The results allowed to distinguish four types (containing two subtypes) characteristic for T. interdigital...

Abstract Injury resolution requires a coordinated immune-mediated response executed by macrophages (MΦs). Yet, how MΦs convert local stimuli into effective repair is poorly understood. Furthermore, organ transplants (Tx) will fail over time due to fibrosis, despite immunosuppressants targeting adaptive immune cells. We hypothesizethis dysregulated MΦ-mediated tissue of grafts. Donor MHCI can be...

This study aims to characterize different Salmonella enterica subsp molecularly. strains (n=49) were isolated from human gastrointestinal cases in the Tolima region and poultry Santander regions using PCR-RFLP, PCR-ribotyping, PCR-SSCP. The band patterns obtained with each technique analyzed by building dendrograms based on Unweighted Pair Group Method Arithmetic mean (UPGMA) method Dice coeffi...

Over the past thirty years, in excess of 36 billion dollars has been invested in the collection of spatial information across the public sector in Europe (PIRA, 2000). There is a clear imperative now to maximise the use of that data captured at such great expense. The information society in which we live makes it ever easier to discover and exploit that data, in principle. However, data collect...