An integrated RNA and CRISPR/Cas toolkit for multiplexed synthetic circuits and endogenous gene regulation in human cells Lior Nissim , Samuel D. Perli , Alexandra Fridkin , Pablo Perez-Pinera, and Timothy K. Lu Department of Biological Engineering and Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA Synthetic Biology Group, Re...

New data are presented for Neotropical Phalangopsidae (Orthoptera, Grylloidea). Four new species are described, Luzara brevipennis Desutter-Grandcolas, n. sp., Miogryllodes hebardi Desutter-Grandcolas, n. sp., Aclella brevipennis Desutter-Grandcolas, n. sp. and Aclella nova Desutter-Grandcolas, n. sp. Nine new recombinations are proposed: Lerneca digrediens (Otte, 2006) n. comb. (Amphiacusta), ...

Phytohemagglutinin (PHA)-induced skin swelling response is widely used as a rough surrogate of integrative cell-mediated and innate immunity across multiple vertebrate taxa due to its simplification and feasibility. However, little is known whether there are sex and interspecific differences of immune responsiveness to PHA in ectotherms, especially for anurans. Therefore, we studied sex and spe...

The costs of caring for offspring are predicted to lead an evolutionary conflict between parents, where each parent benefits if the other provides most care. However, in many biparental species, breeding partners remain together multiple attempts and their respective future reproductive fitness prospects therefore intertwined. Since increase current care by a long-term partner reduces that part...

Photographic identification of individual animals is a non-invasive and cost-effective method that can provide demographic information on wild populations. This study aims to compare two photo-matching algorithms (Wild- ID I3S-Spot) using reintroduced population pool frogs (Pelophylax lessonae) in the UK as case study. We compared following parameters 1) sex age, 2) image quality, 3) collection...

Abstract In monomorphic species, like in the Black-headed Gull, both sexes look alike breeding plumage. With large sets of data on captured and photographed birds using Underhill–Zucchini moult model, we provided a detailed pattern plumage development this species by age sex. This study, similar to other studies, documented first adults with initial stage head at beginning January, yet mean sta...

This paper adopts the PM2.5 concentration data obtained from 1497 station-based monitoring sites, population and gross domestic product (GDP) census data, revealing population exposure and economic effects of PM2.5 in four typical urban agglomerations of China, i.e., Beijing-Tianjin-Hebei (BTH), the Yangtze River delta (YRD), the Pearl River delta (PRD), and Chengdu-Chongqing (CC). The Cokrigin...

Pungentus porosus n. sp. is described and illustrated. The differential characters are a short odontostyle of 6-8 mum or slightly less than one head width, vulva at 44-50%, goblet-shaped amphids, presence of a prevulval uterine sac, and a hemispherical tail with a variable number of pores. Pungenlus sublatum, P. vesiculosus, P. orthocephalus, P. granosus, and P. parvus sensu Eroshenko are consi...