Kinoshita, Mine, Eisuke Kume, Shigeki Igarashi, Nobuko Saito, and Hiroshi Narita. Role of salivary mucin in the protection of rat esophageal mucosa from acid and pepsin-induced injury. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G796–G800, 1999.—The mucosal defensive mechanisms of the esophagus against acid and pepsin remain to be elucidated. In the present study, we investigated the...

Acid gastro-oesophageal reflux (GOR) and gastric aspiration have been labelled as risk factors for chronic rejection bronchiolitis obliterans syndrome (BOS) after lung transplantation (LTx). The present study aimed to further characterise GOR (both acid and nonacid) and the degree of gastric aspiration in LTx recipients both with and without BOS. Impedance-pH recordings were used for GOR detect...

1. A method is described for the preparation of pepsinogen from swine gastric mucosae which consists of extraction and fractional precipitation with ammonium sulfate solutions followed by two precipitations with a copper hydroxide reagent under particular conditions. Crystallization as very thin needles takes place at 10 degrees C., pH 5.0 and from 0.4 saturated ammonium sulfate solution contai...

The objective of this study was to investigate the yield and characteristics collagen protein extracted from muscle four different species mantis shrimp: Miyakella nepa, Harpiosquilla harpax, Erugosquilla woodmasoni, Odontodactylus cultrifer. Mantis shrimp by using a pepsin-solubilization technique, with 0.5 M acetic acid 5% pepsin enzyme. highest M. nepa (0.478 ± 0.06%), which significantly gr...

Isolation of mono-iodotyrosine from slightly iodinated pepsin has been repeated and the properties of the product compared with those of synthetic dl-3-iodotyrosine. Ultraviolet absorption spectra, pK values of the phenol group, solubility measurements, and partition coefficients were so nearly identical for the two materials that there is now no reason to doubt that the product from pepsin is ...

The digestibility of proteins in red kidney bean (Phaseolus vulgaris L.) was examined by in vitro pepsin assay. A 20-kDa polypeptide that remained highly stable after heat processing was identified as a basic subunit of legumin. The results of a monobromobimane (mBBr) labeling test implied that this protein contained rigid intramolecular disulfide bonds, which might contribute to pepsin resista...

OBJECTIVE To examine whether in vitro exposure of mouse middle ear epithelial cells (mMEECs) to conditions that mimic physiologic reflux upregulates Muc5b gene expression and alters cell viability. DESIGN In vitro mMEEC model. SETTING Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC. PARTICIPANTS Cells from the immortalized mMEEC line. MAIN OUTCOM...

Platelet adhesion to collagens immobilized on plastic has been measured, with the following results. (1) Human, but not rabbit, platelets adhered readily to pepsin-extracted monomeric collagens in an Mg2(+)-dependent manner. (2) Rabbit platelets adhered to a monomeric collagen extracted without pepsin by a process that was cation-independent; human platelet adhesion to this collagen exhibited a...