We generated Prx1CreER-GFP transgenic mice that express tamoxifen-inducible Cre recombinase and GFP under the control of a 2.4 kb Prx1 promoter. The transgene is expressed in osteochondro progenitor cells in the developing limb buds and in a subpopulation of periosteal cells that is closely associated with the cortical bone. GFP-expressing cells isolated from the diaphyses of long bones by cell...

Periosteal chondroma is a relatively rare benign cartilaginous neoplasm usually seen in young adults. We presented three cases of periosteal chondroma in the proximal tibia of a 7-year-old girl (Case 1), a 12-year-old boy (Case 2) and a 15-year-8-month old boy (Case 3). Meticulous analysis of initial and follow-up plain radiographs, Computed Tomography (CT) and Magnetic resonance imaging (MRI) ...

− This paper introduces a novel electrochemical detection method for measuring the formation of an immunological complex. The described electrochemical immunoassay consists of several consecutive steps. First the sample is mixed with silver colloid and magnetic particles both previously conjugated to appropriate antibodies. The analyte from the sample binds to both silver and magnetic beads for...

Anodic stripping voltammetry (ASV) with rotating glassy carbon electrode (RGCE) and microfiber carbon electrode (μFCE), both filmed with mercury, were used to determine copper content in wine. Influence of phenolics on in situ mercury film formation was studied. Wine and quercetin were added gradually into solution of Hg(II) in acetic acid-sodium acetate buffer (pH = 4.60). Reduction of Hg(II) ...

15 The electroanalytical technique Absence of Gradients and Nernstian Equilibrium 16 Stripping (AGNES) has been extended by applying stripping chronopotentiometry 17 (SCP) as the reoxidation stage in the determination of the free concentration of Zn 2+ , 18 Cd 2+ and Pb 2+ . This new approach, called AGNES-SCP, has been implemented with 19 screen-printed electrodes (SPE) and the standard Hangin...

Air-stripping method was used to remove ammonia from the wastewater collected from natural gas fertilizer factory. Different materials were used as packing materials for the air stripping system. The effect of pH over 10.5, air-water flow ratio, nature of packing materials, height of materials and initial influent concentration of ammonia on air stripping unit were investigated. An attempt has ...

Ants algorithm is a universal and flexible solution which was first designed for solving optimization problem such as Traveling Salesman Problem. Analogy between finding the shortest way by ants and finding documents most alike, became a stimulus of ant based text document clustering method. This method consist of two phases, which are finding documents most alike (trial phase) and clusters mak...

Early diagnosis of osteomyelitis is of paramount importance to avoid irreversible sequelae. This often requires a multimodal approach. The purpose of the present study was primarily to evaluate how ultrasound might be useful in early diagnosis of osteomyelitis in the paediatric age group. The evolution of ultrasound findings with progression and resolution of disease was also analysed. In this ...

BACKGROUND The corticoperiosteal flap from the medial femoral condyle has become the workhorse in the management of recalcitrant nonunions with vascularized periosteum. Inclusion of the outer condylar cortex has been advocated to avoid damaging the osteogenic capacity of the periosteum and is at present an ordinary technical step in the procedure. PATIENTS A clinical prospective study was und...

Apert syndrome (AS), a severe form of craniosynostosis, is caused by dominant gain-of-function mutations in FGFR2. Because the periosteum contribution to AS cranial pathophysiology is unknown, we tested the osteogenic potential of AS periosteal cells (p.Ser252Trp mutation) and observed that these cells are more committed toward the osteoblast lineage. To delineate the gene expression profile in...