Glutamic proteases are a distinct, and recently re-classified, group of peptidases that are thought to be found only in fungi. We have identified and analysed the distribution of over 20 putative glutamic proteases from all fungal species whose genomes have been sequenced so far. Although absent from the Saccharomycetales class, glutamic proteases appear to be present in all other ascomycetes s...

A manganese peroxidase gene (mnp1) from Phanerochaete chrysosporium was efficiently expressed in Aspergillus oryzae. Expression was achieved by fusing the mature cDNA of mnp1 with the A. oryzae Taka amylase promoter and secretion signal. The 3' untranslated region of the glucoamylase gene of Aspergillus awamori provided the terminator. The recombinant protein (rMnP) was secreted in an active fo...

The manganese peroxidase (MnP) of Phanerochaete chrysosporium supported Mn(II)-dependent, H202independent lipid peroxidation, as shown by two findings: linolenic acid was peroxidized to give products that reacted with thiobarbituric acid, and linoleic acid was peroxidized to give hexanal. MnP also supported the slow oxidation of phenanthrene to 2,2'-diphenic acid in a reaction that required Mn(...

-Among 12 basidiomycetaceous fungal strains, Phanerochaete chrysosporium SC26 and K3 and Phanerochaete flavido-alba FPL 106507 were the most efficient in decolorizing paper mill wastes. Enzymatic activities attributable to both families of extracellular ligninases, lignin peroxidase (LIP) and manganese peroxidase (MnP), have been detected in P. flavido-alba FPL 106507. Both peroxidase activitie...