For the discovery of novel chemical matter generally endowed with bioactivity, strategies may be particularly efficient that combine previous insight about biological relevance, e.g., natural product (NP) structure, methods enable coverage space, such as fragment-based design. We describe de novo combination different 5-membered NP-derived N-heteroatom fragments to structurally unprecedented “p...

Phenotypic screening is a powerful technique that allowed the discovery of antimicrobials to fight infectious diseases considered deadly less than century ago. In high throughput phenotypic assays, thousands compounds are tested for their capacity inhibit microbial growth in-vitro . After an active compound found, identifying molecular target next step. Knowing specific key understanding its me...

The Antivirogram and PhenoSense assays are widely used phenotypic tests for HIV drug resistance. There are limited data on the reproducibility of each assay, and little is known about the correlation between the two. Using data from the Stanford HIV drug resistance database, we performed a comprehensive analysis of the reproducibility of each assay, and calculated the correlation and concordanc...

Taxonogenomics combines phenotypic assays and genomic analysis as a means of characterizing novel strains. We used this strategy to study Marseille-P9898T strain, an aerobic, motile, Gram-negative, spore-forming, rod-shaped bacterium isolated from planarian Schmidtea mediterranea. is catalase-positive oxidase-negative. The major fatty acids detected are 12-methyl-tetradecanoic acid, 13-methyl-t...

Background Infertility affects around 15% of couples, and male factors account for 50%. Cryptorchidism is one of the most common causes for azoospermia. Generation of functional spermatids from azoospermia patients is of unusual significance for treating male infertility. It has been recently reported by peers and us that human spermatogonial stem cells (SSCs) can be clearly identified and cult...

The study sought to determine the rate of discordant results between genotypic and phenotypic tests for diagnosis drug-resistant tuberculosis (DR-TB). Sputum samples cultured isolates from suspected DR-TB patients were, respectively, analyzed Mycobacterium by Xpert® MTB/RIF (Cepheid, Sunnyvale, CA, USA) line probe assays (LPA) (Hain, Nehren, Germany). Discrepant rifampicin (RMP)-resistant were ...