MEYER, JAMES H., AND MORTON I. GROSSMAN. Comparison of Dand L-phenylalanine as pancreatic stimulants. Am. J. Physid. 222(a) : 1058-1063. 1972.-In dogs with pancreatic fistulas and arrangements for perfusing intact intestine, the following observations were made: 1) during continuous intravenous infusion of a submaximal dose of secretin, instillation of L-phenylalanine at pH 7 into the duodenum ...

Mutant strains of Escherichia coli have been isolated in which the synthesis of 3deoxy-D-arabinoheptulosonic acid 7-phosphate (DAHP) synthetase (phe) is derepressed, in addition to those enzymes of tyrosine biosynthesis previously shown to be controlled by the gene tyrR. The major enzyme of the terminal pathway of phenylalanine biosynthesis chorismate mutase-prephenate dehydratase is not derepr...

Although 3-phenylserine, which is an antagonist of phenylalanine, has been known to be degraded by phenylserine aidolase,i) serine hydroxymethyltransferase,2) and L-amino acid oxidase,i) little attention has been paid to the metabolism of 3-phenylserine in microorganisms. We have found that D-threo-3-phenylserine was a better substrate for NADP'-dependent D-threonine dehydrogenase [EC 1.1.1.-] ...

The electron spin echo envelope modulation (ESEEM) investigation of the high-pH (hpH) form of sulfite oxidase (SO) and sulfite dehydrogenase (SDH) prepared in buffer enriched with H(2)(17)O reveals the presence of three types of exchangeable oxygen atoms at the molybdenum center. Two of these oxygen atoms belong to the equatorial OH ligand and the axial oxo ligand, and are characterized by (17)...

High temperature is one of the most important environmental factors that affect the successful cultivation of apple (Malus × domestica Borkh). The expression of genes related with anthocyanin synthesis and sugar accumulation by high temperature treatment was investigated in the fruits of “Fuji” apple at different developmental stages in different temperature conditions through realtime PCR. In ...

The effects of ulinastatin (ULN), a human urinary protease inhibitor, on liver injury caused by ischemia-reperfusion were studied in rats. In the liver ischemia-reperfusion model, ULN suppressed the elevation of serum transaminase levels and tissue lipid peroxide levels in the liver. ULN did not exhibit a radical-trapping action on the superoxide and hydroxyl radicals as measured by electron sp...

Lignin is one of the major characteristics of plant secondary cell wall that provides structural rigidity for the cells and tissues and hydrophobicity to tracheary elements. Internode tissues of Aeluropus littoralis as a halophyte grass were sampled at different developmental stages (from the first to the fifth internodes ) and under different NaCl concentrations. The influences of NaCl and int...

We describe a method based on the traditional arteriovenous balance technique in conjunction with muscle biopsies for the determination of leg muscle protein balance during the nonsteady state in blood amino acid concentrations. Six young, healthy individuals were studied in the postabsorptive state (pre-Phe) and after a bolus ingestion of approximately 0.5 g phenylalanine (post-Phe). Post-Phe ...

The rate of release of deuterons into the body water from 2,3,4,5,6-pentadeutero-L-phenylalanine has been shown to be a valid measure of the activity of the phenylalanine hydroxylase system in vivo. At a dose of 0.5 g/kg, the rate of release of deuterons is linear for 60 to 90 min. Male rats, which had previously been shown to have 22 to 25% more phenylalanine hydroxylase activity in liver extr...

Compartmentalization of amino acid was investigated to define conditions required for accurate measurements of rates of protein synthesis in rat lungs perfused in situ. Lungs were perfused with Krebs-Henseleit bicarbonate buffer containing 4.5% (w/v) bovine serum albumin, 5.6mm-glucose, normal plasma concentrations of 19 amino acids, and 8.6-690mum-[U-(14)C]phenylalanine. The perfusate was equi...