Choline oxidase from Arthrobacter globiformis is a flavin-dependent enzyme that catalyzes the oxidation of choline to betaine aldehyde through two sequential hydride-transfer steps. The study of this enzyme is of importance to the understanding of glycine betaine biosynthesis found in pathogenic bacterial or economic relevant crop plants as a response to temperature and salt stress in adverse e...

The botanist Vines believed that he was able to separate both peptone-forming and peptone-splitting enzymes from the milky sap of Carica papaya (1). Applying quantitative methods to the hydrocyanic acid activation of papain, Willstatter and Grassmann (2) concluded that papain is a homogeneous enzyme. They assumed that hydrocyanic acid plays the role of a kinase that extends the specificity rang...

Dehydrogenase activities in red blood cells of rabbits made anemic with phenyihydrazinc were assayed. These assays were continued at the time of reticulocytosis and followed through the course of in vivo maturation of the reticulocytes to a younger erythrocyte population. Isocitric, glucose-6-phosphate, and 6-phosphogluconic dehydrogenases were more active in the reticulocytes. Isocitric and gl...

By MARCEL E. CONRAD, LEWIS R. WEINTRAUB AND WILLIAM H. CROSBY T HE QUANTITY of iron present in the body is regulated by a balance between iron absorption and loss.15 Iron-deficient and iron-loaded animals and humans attempt to re-establish a normal body store of iron by regulating absorption16 and a limited but selective loss of body iron.58 The hemolytic states are unusual in that increased ab...

THE ONLY METHOD available at present for the routine determination of citrulline in blood is the one first described by Archibald (1). This method is based on the original observation of Fearon (2) that in strongly acid solution citrulline condenses with diacetylmonoxime (DAM) to produce a peach-colored complex. Since urea also reacts with DAM under these conditions, Archibald removed it by inc...

The mechanism of induction of anisocytosis was studied with experimental anemia of rabbits induced by blood depletion and phenylhydrazine chloride injection. The Price-Jones’ curves of erythrocytes from anemic animals showed a large variety of red cell size, indicating the appearance of abnormally large sized cells. RNA contents of some reticulocytes in anemia were comparable to those of polych...

Reaction of equimolar equivalents 1-(5-methyl-1-(4-nitrophenyl)-1H-1,2,3-triazol-4-yl)ethan-1-one (1) and N-phenylhydrazinecarbothioamide (2) in boiling ethanol containing a catalytic amount concentrated hydrochloric acid for 4 h gave (Z)-2-(1-(5-methyl-1-(4-nitrophenyl)-1H-1,2,3-triazol-4-yl)ethylidene)-N-phenylhydrazine-1-carbothioamide (3) with 88% yield. The structure 3 was established usin...

Dapsone hydroxylamine (DDS-NOH) is a direct-acting hemolytic agent responsible for dapsone-induced hemolytic anemia in the rat. The hemolytic activity of DDS-NOH is associated with the formation of disulfide-linked hemoglobin adducts on membrane skeletal proteins. We have postulated that this membrane protein "damage" is a consequence of DDS-NOH-induced oxidative stress within the red cell and ...

The prophage-inducing capability of hydroxylamine sulfate and 36 of its derivatives, and of hydrazine dihydrochloride and dihydrazine sulfate and 43 of their derivatives, was determined in Escherichia coli W1709 (X). Maximal nontoxic concentrations up to 1 mg/ml were tested. Hydroxylamine sulfate was active at 2.5 ,ug/ml and the following 17 derivatives were active at concentrations ranging up ...

The maturation of reticulocytes into erythrocytes was demonstrated in vitro. Reticulocytosis was induced in rats by repeated bleeding or by phenylhydrazine injections. Whole blood samples were then incubated for 2 days at 37 degrees C. Reticulocytes in culture changed from polylobulated, monoconcave or triconcave forms to biconcave disks. During the first 12 h in vitro, the average reticulocyte...