The binding of Escherichia coli and Lactococcus lactis Fapy-DNA glyosylase (Fpg) proteins to DNA containing either cyclic or non-cyclic abasic (AP) site analogs was investigated by electrophoretic mobility shift assay (EMSA) and by footprinting experiments. We showed that the reduced AP site is the best substrate analog for the E.coli and L.lactis enzymes ( K Dapp = 0.26 and 0.5 nM, respectivel...

Adenosine receptor antagonists initiate repetitive bursting activity in the CA3 region of hippocampal slices. Although some studies have suggested that this effect is irreversible, this has been difficult to establish because many adenosine antagonists wash out of brain slices extremely slowly. Furthermore the cellular mechanism that underlies persistent bursting is unknown. To resolve these is...

Decarbomethoxylated JW062 (DCJW), the active component of the oxadiazine insecticide (S)-methyl 7-chloro-2,5-dihydro-2-[[(methoxycarbonyl)[4-(trifluoromethoxy)phenyl] amino]carbonyl] indeno[1,2-e][1,3,4]oxadiazine-4a(3H)-carboxylate (DPX-JW062) (indoxacarb), was tested on 2 inward voltage-dependent sodium currents (named INa1 and INa2) expressed in short-term cultured dorsal unpaired median neu...

We studied gamma-aminobutyric acid (GABA)-mediated currents in short-term cultured dorsal unpaired median (DUM) neurons of cockroach Periplaneta americana using the whole cell patch-clamp technique in symmetrical chloride solutions. All DUM neurons voltage-clamped at -50 mV displayed inward currents (I(GABA)) when 10(-4) M of GABA was applied by pneumatic pressure-ejection pulses. The semi-loga...

This document extends the main paper by presenting a more in-depth analysis of the temporal clustering approach proposed in Sec. 5.2 in the paper, expanding the validation paragraph of Sec. 7 in the paper, and presenting screenshots for the results shown in the supplementary video. 1. Temporal Clustering Algorithm. Sec. 5.2 in the main paper introduces a temporal clustering approach that divide...

The mechanism of charge transport through Nafion polymers coated on glassy carbon electrodes and containing Cp2FeTMA+, R~(bpy),~+, and O~(bpy),~+ (where Cp2FeTMA+ is [(trimethylammonio)methyl]ferrocene, bpy = 2,2'-bipyridine) is described. The apparent diffusion coefficients, Dam,, of polymer modified electrodes, as measured by conventional electrochemical methods and which can include contribu...

INTRODUCTION In peripheral nerve injury, nervous fibers originate and grow from the proximal stump (1). In digital nerves injuries, the best outcomes after repair were found in children, showing the need of further nervous regeneration studies in adult populations(2). Using a tube for repairing an injured nerve is not new (3). Tubes seem to be important for regeneration because they comprise ne...

Ionized calcium (Ca2+) is an intracellular messenger in nearly all eukaryotic organisms. Calcium waves have been observed in many cell types, including airway epithelia, eggs, hepatocytes, exocrine pancreas, cultured glial cells, and neuronal cells. These waves travel across or between cells without slowing or getting smaller, and so cannot be generated by pure passive diffusion. Rather, the wa...

A UV-curable, flame-retardant monomer, DAPP (bis[di-acryloyloxyethyl]-p-tert-butyl-phenyl-phosphate), was synthesized based on BPDCP (4-tert-butylphenyl-dichloro phosphate) and HEA (2-hydroxy ethyl acrylate). DAPP was blended with regular bisphenol A epoxy acrylate (BAEA) in various ratios to yield various phosphorus contents. The TGA-IR (thermogravimetric analyzer interface with an infrared sp...