OBJECTIVE The aim of this study was to evaluate the influence of exposure of the mineral trioxide aggregate (MTA) - with and without calcium chloride (CaCl2) - to phosphate-buffered saline (PBS) on the apical microleakage using a glucose leakage system. MATERIAL AND METHODS Sixty root segments were randomly divided into 4 experimental groups (n=15). After resecting the apical segments and enl...

Nowadays antibiotics are used on a large scale in veterinary and human medicine to cure or prevent diseases. Some antibiotics injected into hatching eggs to eliminate pathogens and prevention of egg transmission of disease. Adverse effects of drugs have always been a major concern. There is scantly information available about the safety and pathological alterations of lincosamide-aminocyclitol ...

An overview of the formation of calcium phosphate under in vitro environment on the surface of a range of bioactive materials (e.g. from silicate, borate, and phosphate glasses, glass-ceramics, bioceramics to metals) based on recent literature is presented in this review. The mechanism of bone-like calcium phosphate (i.e. hydroxyapatite) formation and the test protocols that are either already ...

Fibroblast growth factor receptor 3 (FGFR3) is a transmembrane tyrosine kinase protein in the fibroblast family, which plays key role many biological processes. Over-expression and activating mutations FGFR3 are frequent non-invasive bladder cancer, highlighting this as potential biomarker for recurrent cancer detection. Affimer reagents isolated against recombinant were assessed their affinity...

nowadays antibiotics are used on a large scale in veterinary and human medicine to cure or prevent diseases. some antibiotics injected into hatching eggs to eliminate pathogens and prevention of egg transmission of disease. adverse effects of drugs have always been a major concern. there is scantly information available about the safety and pathological alterations of lincosamide-aminocyclitol ...

We have developed a model to study the neoplastic transformation of rat oligodendrocytes in culture. This procedure utilizes a technique previously developed by McCarthy and de Vellis which allows the preparation of 99% pure astrocyte and oligodendrocyte populations from 1- to 2-day-old rat cerebral cortices. Pregnant rats on the 19th day of gestation were given injections with either ethyl nit...

In this study, trypsin-like protease activity and expression of serine encoding genes (PmPPAE1, PmPPAE2 - clip-serine proteases PmTLP1, PmTLP2 proteases) in the different tissues black tiger shrimp were determined. The level PmPPAE1 was highest hemolymph; meanwhile that PmTLP1 hepatopancreas. hemolymph white spot syndrome virus (WSSV)-injected shrimp, PmPPAE1, upregulated by 2.32, 2.57 4.56-fol...

A flow cytometric method for the quantitation of reticulocytes was refined for routine laboratory use. Blood (2 microliters) is added to 2 ml of 0.4 microM thiazole orange in phosphate buffered saline, incubated at room temperature for 90 minutes, and analysed on a Coulter EPICS Profile flow cytometer, with gating for red cells on the basis of forward and right angled light scatter. Blood (2 mi...

Two irritants, phosphate-buffered saline and alcohol, and antigens including killed Brucella abortus, live B. abortus, Staphylococcus aureus, and rat parvovirus were separately infused into rat mammary glands during pregnancy, and by using immunofluorescent techniques, the numbers of immunoglobulin-containing cells in glands during lactation and involution were determined. The study provided ba...