An inositol-requiring strain of Neurospora crassa was labelled during growth in liquid medium with [3H]inositol, and the levels of inositol phosphates and phosphoinositides were determined under inositol-sufficient and inositol-starved conditions. Because the mutant has an absolute requirement for inositol, the total mass of inositol-containing compounds could be determined. Inositol-containing...

Biofortification of commonly eaten staple food crops with essential mineral micronutrients is a potential sustainable solution to global micronutrient malnutrition. Because phytic acid (PA; 1,2,3,4,5,6-hexakis myo-inositol) reduces mineral micronutrient bioavailability, reduction of PA levels could increase the bioavailability of biofortified iron (Fe), zinc (Zn), calcium (Ca), and magnesium (M...

Acid soils contain elevated concentrations of mobile aluminium (Al) ions which are toxic for plants. Plants form symbioses with the rhizosphere microorganisms stimulating plant growth and affecting Al availability. Here, first time approach based on ability to immobilize in soil was applied initial selection beneficial microorganisms. Al-Immobilizing yeast Rhodotorula sp. AL1 12 bacterial strai...

Reagent grade chemicals were used where available. Adenosine triphosphate and coenzyme A were obtained from the Pabst Laboratories; riboflavin-5'-phosphate was obtained from the California Foundation for Biochemical Research; sugar phosphates were obtained from Nutritional Biochemicals Corporation. "Levigated Alumina," A-542, from Fisher Scientific Company, was used in preparing ground-cell sus...

Abstract To fabricate a novel metallosilicate zeolite with tridirectional pore system of 8- and 10-ring channels, direct incorporation B, Al Ga into small medium ITQ-52 the IFW-type topology was carried out using phosphorus-containing organic structure-directing agent (P-OSDA, 1,4-butanediylbis[tris(dimethylamino)]phosphonium hydroxide). The solids were characterized by XRD, FT-IR, 11B, 27Al, 3...

A variety of metabolic intermediates of the glycolytic pathway and tricarboxylic acid cycle have been found to be inhibitors of 6-phosphogluconate dehydrogenase isolated from sheep liver. All nucleoside 5’-triphosphates and 5’diphosphates are inhibitors with Ki values averaging 0.8 mM, nucleoside 5’-monophosphates are also inhibitory with Ki values averaging 6 mu. The 2’and 3’monophosphates of ...

Administration of anethole at 10 and 50 mg doses caused significant reduction in seminal vesicle weight, RNA and protein concentrations and contents and acid phosphates activity. However, there was significant increase in DNA concentrations and in the activities of alkaline phosphates and lactate dehydrogenase. The effects were more pronounced in the group receiving 50 mg of anethole.

Chemical and enzymatic procedures have been employed for the preparation of various phosphorylated derivatives of the acyclonucleoside 9-(1,3-dihydroxy-2-propoxymethyl)adenine, an analogue of the active antiviral agent 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG). In combination with the previously reported 2',3'-seco nucleosides and their phosphates and cyclic phosphates (Stolarski et al., ...