Lysophosphatidic acid (LPA) and the LPA-generating enzyme autotaxin (ATX) have been implicated in lymphocyte trafficking and the regulation of lymphocyte entry into lymph nodes. High local concentrations of LPA are thought to be present in lymph node high endothelial venules, suggesting a direct influence of LPA on cell migration. However, little is known about the mechanism of action of LPA, a...

Many chemical mediators regulate neutrophil recruitment to inflammatory sites. Although the actions of each chemical mediator have been demonstrated with neutrophils in vitro, how such chemical mediators act cooperatively or counteractively in vivo remains largely unknown. Here, by in vivo two-photon excitation microscopy with transgenic mice expressing biosensors based on Förster resonance ene...

Embryonic cells that migrate long distances must critically balance cell division in order to maintain stream dynamics and population of peripheral targets. Yet details of individual cell division events and how cell cycle is related to phases of migration remain unclear. Here, we examined these questions using the chick cranial neural crest (NC). In vivo time-lapse imaging revealed that a typi...

In many immunological processes chemoattraction is thought to play a role in guiding cells to their sites of action. However, based on in vivo two-photon microscopy experiments in the absence of cognate antigen, T cell migration in lymph nodes (LNs) has been roughly described as a random walk. Although it has been shown that dendritic cells (DCs) carrying cognate antigen in some circumstances a...

background: the purpose of this study was to investigate the various gantry angle and ssd dependencies of tld and mosfet dosimeters. materials and methods: lif (mg) tld and mosfet were used in this study. dosimeter systems were calibrated and then irradiated at various gantry angle and ssd by applying 6 mv photon energy. results: based on the results, mosfet changes were found to be in 2% range...

Background: Skyshine describes the radiation scattered by the atmosphere above a LINAC facility to a point on the ground. The aim of this study was to measure the skyshine photon dose rates from two different (9 MV and 18 MV) medical linear accelerators. Materials and Methods: The photon beam was directed upward (180° gantry position), with a maximum photon field size (40 × 40 cm2) at the isoce...