AbstractIn the last step of desolvation method for preparation of albumin nanoparticles, glutaraldehyde (GA) is added to stabilize the newly formed nanoparticles. Due to undesirable effects of GA, the objective of this study was to evaluate alternative methods of crosslinking including ultraviolet (UV) irradiation, adding of glucose and combination of both methods. The nanoparticles were prepar...

The use of collagen scaffold in tissue engineering is on the rise, as modifications to mechanical properties are becoming more effective in strengthening constructs whilst preserving the natural biocompatibility. The combined technique of plastic compression and cross-linking is known to increase the mechanical strength of the collagen construct. Here, a modified protocol for engineering these ...

The present review aims to give a closer look of hydrogels based on chitosan and poly (vinyl alcohol) and to discuss their potential biomedical applications in drug delivery system. Various investigations based on chitosan/poly (vinyl alcohol) carried out recently by researchers have been reported in this review. Moreover different chemical and physical crosslinking methods used for hydrogels f...

OBJECTIVES The purpose of the present study was to evaluate the effects of proanthocyanidin (PAC), a crosslinking agent, on the physical properties of a collagen hydrogel and the behavior of human periodontal ligament cells (hPDLCs) cultured in the scaffold. MATERIALS AND METHODS Viability of hPDLCs treated with PAC was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium b...

Functional tissue engineering of load-bearing repair tissues requires the design and production of biomaterials that provide a remodelable scaffold for host infiltration and tissue regeneration while maintaining the repair function throughout the remodeling process. Layered constructs have been fabricated from chemically and mechanically cleaned porcine intestinal collagen using ethyl-3(3-dimet...

The purpose of the present study was to design muco-adhesive chitosan microspheres containing amoxicillin. Chitosan microspheres with a small particle size and good sphericity were prepared by a spray-drying method followed by chemical treatment with a chemical crosslinking agent (glutaraldehyde). Parameters affecting the crosslinking extent of the crosslinking time and the concentration of the...

BACKGROUND Injectable, acellular biomaterials hold promise to limit left ventricular remodeling and heart failure precipitated by infarction through bulking or stiffening the infarct region. A material with tunable properties (eg, mechanics, degradation) that can be delivered percutaneously has not yet been demonstrated. Catheter-deliverable soft hydrogels with in vivo stiffening to enhance the...

Abstract Recent years have seen major advances in the additive manufacturing of stimuli‐responsive materials, also known as “4D printing,” among which liquid crystal elastomers (LCEs) play an important role. However, during fabrication photo‐crosslinking LCEs is required, but this step time‐consuming and efficient polymerization challenging, especially case light‐responsive materials. In work, ...

Tumors are characterized by extracellular matrix (ECM) remodeling and stiffening. The importance of ECM remodeling to cancer is appreciated; the relevance of stiffening is less clear. We found that breast tumorigenesis is accompanied by collagen crosslinking, ECM stiffening, and increased focal adhesions. Induction of collagen crosslinking stiffened the ECM, promoted focal adhesions, enhanced P...

Functional crosstalk between G protein-coupled receptors in a homo- or heterodimeric assembly likely involves conformational changes at the dimer interface, but the nature of this interface is not yet established, and the dynamic changes have not yet been identified. We have mapped the homodimer interface in the dopamine D2 receptor over the entire length of the fourth transmembrane segment (TM...