Pigeonpea is an important grain legume, however the genetic transformation of pigeonpea has been very challenging to date. In the present study, we describe a simple protocol for plantlet regeneration via multiple shoot induction from 12-16 day old immature embryos of pigeonpea cultivar ICPL 87 which can be used for efficient transformation. By optimizing the culture medium, we have achieved or...

In vitro plantlet regeneration has been obtained from 20-25days old seedling shoot tip segments of Cucumis melo (L.). We were examined using various phyto hormones individually and in combination on Murashige and Skoog (MS) semi solid medium supplemented with BAP (1.0-3.0 mg/L) Kn (1.0-3.0 mg/L),IAA (0.5 mg/L)+ BAP (1.0-3.0 mg/L) and IAA (0.5 mg/L)+Kn (1.0-3.0 mg/L) for multiple shoots prolifer...

Embryogenesis in cotton is a difficult task due its genome dependency. We used 3 cotton cultivars (Khandwa-2, G. Cot. 10, and BC-68–2) and Coker-312 as control for regeneration. Efficient somatic embryogenesis was induced in agronomically important Indian cotton cultivars, Khandwa-2 and G. Cot. 10. For callusing in all the cultivars, different media combinations were tried. Embryogenesis was in...

Seeds of Bauhinia acuminata L. were used as an experimental material for micropropagation. The effect of growth regulators concentrations on morphogenetic development was studied. Seeds were germinated on MS medium supplemented with GA3 (0.10, 1.0 and 2.0 mgL -1 ) and without GA3. Satisfactory germination was observed at media with 1.0 mgL -l GA3. Subsequent propagation from plantlet was perfor...