The present study was undertaken to investigate the role of banana extract (BE) on synchronous plantlet formation from seeds of Dendrobium lituiflorum Lindl. The seedlings formed on modified Knudson C (KC) medium supplemented with 20% (v/v) BE had significantly high fresh and dry weights. Morphologically uniform plantlets with elongated leaves and well developed roots were formed on KC medium w...

Here, we present the draft genome of Bacillus velezensis 3A-25B, which totaled 4.01 Mb with 36 contigs, 3,948 genes, and a GC content of 46.34%. This strain, which demonstrates biocontrol activity against root rot causal phytopathogens in horticultural crops and friendly interactions in roots of pepper plantlets, was obtained from grassland soil in Zacatecas Province, Mexico.

Context: It is necessary to focus on the importance of adopting micropropagation technique for mass propagation of the plantlets in commercial scale as well as conservation and distribution of germplasm. Objective: The present investigation has been designed with a view to establishing protocol of in vitro regeneration of medicinal plant species i,e., Vitex nigundo L (Verbenaceae). Materials an...

Using an RNA sequencing (RNA-seq) approach, we analyzed the differentially expressed genes (DEGs) and physiological behaviors of "Manicure Finger" grape plantlets grown in vitro under white, blue, green, and red light. A total of 670, 1601, and 746 DEGs were identified in plants exposed to blue, green, and red light, respectively, compared to the control (white light). By comparing the gene exp...

The present studies report in vitro propagation of a rare and medicinally important orchid Vanda testacea through foliar explants. Explants were cultured on Mitra et al. (M) medium and its combinations with cytokinins (BAP, Kn 1 mgl each) and auxin (NAA 1 mgl) individually and in combination for initiation of regeneration response, proliferation of regenerants and subsequent development of plan...

Two methods were developed for the generation of cisgenic apples. Both have been successfully applied producing trees. The first method avoids the use of any foreign selectable marker genes; only the gene-of-interest is integrated between the T-DNA border sequences. The second method makes use of recombinase-based marker excision. For the first method we used the MdMYB10 gene from a red-fleshed...

Abstract The objective of this work was to evaluate the effects indolebutyric acid (IBA) concentration, substrate composition and volume, propagule type on adventitious rooting quality Cordia trichotoma plantlets produced by mini-cuttings. Mini-cuttings were evaluated for percentage root number length at 30 60 days cultivation. Plantlets shoot height stem diameter 30, 60, 90, 120 Dickson index,...

The aim of this study was to produce sugarcane plantlets from cell suspension culture and study its genetic fidelity using molecular markers. The study was carried out using sugarcane varieties Co 86032 and Q117. Callus cultures of both the varieties were optimized using six different callus induction media. After screening the growth response of callus on six different callus induction media, ...

Fusariosis (Fusarium guttiforme) is the most destructive disease of pineapple crop in Brazil. The aim this study was to evaluate reaction an isolate genotypes selected for crownless fruit weight (CFW) and total soluble solids (TSS). A 1,125 ('BRS Imperial'×'Pérola') were evaluated. Selection greatest CFW TSS performed during sexual cycle. inoculated on slip-type plantlets using immersion techni...

In vitro propagation of Cavendish banana is an alternative to produce healthy, uniform and sufficient plant materials high qualities. Appropriate culture media formulation needed for shoot growth rooting stage. This experiment aimed study effects IAA organic supplements on in rooting. Explants were subjected MS medium with various supplements, a factorial arrangement completely randomized desig...