Ex vitro strawberry plantlets from micropropagation and coinoculated with arbuscular mycorrhizal fungi (AMF) biochar can provide beneficial health effects. In the present study, we evaluated effects of different proportions in presence absence AMF on production secondary metabolites leaves roots during ex acclimatization. Additionally, enzymatic activity substrate enriched was analyzed. The exp...

An efficient protocol was developed for the regeneration of plantlets via direct and indirect organogenesis from stem nodal segments of Naravelia zeylanica (L.) DC. (Ranunculaceae). The interaction of 6-benzyl aminopurine (BAP) with -naphthalene acetic acid (NAA) in Murashige and Skoog (1962) medium induced a varied morphological response. A maximum of 17.6 ± 1.5 shoots per explant were obtaine...

micropropagated plantlets derived from three different grape rootstock genotypes namely, dogridge (vitis champini), so4 (v. berlandieri×v. rupestris) and ari-h-144 (v. vinifera×v. labrusca) were subjected to randomly amplified polymorphic dna (rapd) and inter simple sequence repeats (issr) analyses in order to evaluate their genetic stability and/or detect likely existing variations among in vi...

shoot tips from actively growing, greenhouse maintained plants of kalanchoe blossfeldiana were cultured in vitro for shoot proliferation and root initiation on murashige and skoog (ms) basal medium supplemented with naa and ba, both in concentrations of 0.00, 0.50, 1.00 and 2.00 mg l-1. results showed that the maximum plantlets height (7.012 cm), node number (4.516), root number (8.860) and roo...

Brinjal (Solanum melongena L.) var. Mattu Gulla (MG) and var. Perampalli Gulla (PG) are unique varieties with distinct flavour cultivated in Udupi, Karnataka State, and are exposed to several biotic and abiotic stresses. An efficient and reproducible in vitro regeneration method is required to expedite the manipulation of these brinjal varieties to cope up with stress by tissue culture and gene...

Phalaenopsis amabilis (L.) Blume Orchid is one of the species in genus and are well-known as exotic beautiful white flowers. Besides that, there little continuity mass propagation that produce many standardized seedlings a relatively short period to satisfy market demand. This study was focusing on evaluating effect natural additives plant growth regulators (PGR). PGR used nutritional constitue...

To investigate the suitable medium for in vitro shoot regeneration and plantlet growth of Dendrobium crumenatum Sw., individual protocorm-like bodies (PLBs) (about 4 - 5 mm diameter) Sw. derived from MS supplemented with 0.5 mg/L TDZ 60 days culture were cultured on 6 media; Murashige Skoog (MS) medium, 15 % (v/v) CW, CW 0.2 (w/v) AC, Vacin Went (VW) VW AC. After months culture, containing coco...

Plants react differently to various media concentration and constituents. Growth of Senna alata (linn) inoculated on woody plant media (WPM) and Murashige & Skoog (MS) supplemented with growth regulators were investigated. Mature seeds were collected from the medicinal garden of National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan; latitude 70221N; longitude 30 551E. Seeds ...

The application of tissue culture techniques, particularly in the area of embryo rescue, has had a major impact on the maintenance and development of hybrid embryo from wide crosses. Embryo rescue techniques are directed towards obtaining more efficient survival of embryos in situations where very immature embryos are to be cultured. An in vitro embryo rescue culture technique has been develope...