Cells closely coordinate cell division with chromosome replication and segregation; however, the mechanisms responsible for this coordination still remain largely unknown. Here, we analyzed the spatial arrangement and temporal dynamics of the 9.1 Mb circular chromosome in the rod-shaped cells of Myxococcus xanthus. For chromosome segregation, M. xanthus uses a parABS system, which is essential,...

valois (vls) was identified as a posterior group gene in the initial screens for Drosophila maternal-effect lethal mutations. Despite its early genetic identification, it has not been characterized at the molecular level until now. We show that vls encodes a divergent WD domain protein and that the three available EMS-induced point mutations cause premature stop codons in the vls ORF. We have g...

Proteasome-mediated protein degradation is a key regulatory mechanism in a diversity of complex processes, including the control of cell cycle progression. The selection of substrates for degradation clearly depends on the specificity of ubiquitination mechanisms, but further regulation may occur within the proteasomal 19S cap complexes, which attach to the ends of the 20S proteolytic core and ...

Placement of the division site at midcell in Escherichia coli requires the MinE protein. MinE acts by imparting topological specificity to the MinCD division inhibitor, preventing the inhibitor from acting at the midcell site while permitting it to block division at other unwanted sites along the length of the cell. It was previously shown that MinE assembled into a ring structure that appeared...

The present study investigates the crystal preferred orientation (CPO) of quartz crystals in psammitic rocks to ascertain the deformationmechanism using electron backscatter diffraction (EBSD) on quartz crystals from north of the Renvyle-Bofin Slide (RBS) nearLetterfrack in western Ireland. Complete crystallographic orientations were determined for several thousand individual quartz crystalsin ...

Several methods are available for performance prediction of single phase shaded pole induction motors. A simple model, in conjunction with a more complicated and accurate model, which considers the asymmetrical windings and core loss, is used to compare the prediction methods against experimental results. Saturation is incorporated in the model. Fundamental and harmonic flux density wave distri...

Pairing of homologous chromosomes is a crucial step in meiosis, which in fission yeast depends on nuclear oscillations. However, how nuclear oscillations help pairing is unknown. Here, we show that homologous loci typically pair when the spindle pole body is at the cell pole and the nucleus is elongated, whereas they unpair when the spindle pole body is in the cell center and the nucleus is rou...

The paper analyzes global solutions to the optimal digital controller realization problem based on maximizing a finite word length (FWL) closed-loop stability measure. For each closed-loop eigenvalue, a single-pole FWL stability function is first introduced, and a single-pole FWL stability measure is then defined as the maximum of the corresponding single-pole stability function over all the co...

The pole vaulting is a sport with a long history involving complex dynamic motion. The aim of pole vaulting for the athlete is to achieve maximum height. The dynamic behavior of the pole-athlete system is identical to an inverted pendulum system with the buckling pole acting as a spring. The purpose of this paper is to study the influence of factors on the pole vaulting performance such as the ...