Dot blot and transblot enzyme-linked immunosorbent assays (e.l.i.s.a.) are described which provide sensitive non-radioactive methods for screening Z-DNA-specific antisera and for detecting Z-DNA in polydeoxyribonucleotides and supercoiled plasmids. In the alkaline phosphatase dot blot e.l.i.s.a., Z-DNA, Br-poly(dG-dC).poly(dG-dC), or B-DNA, poly(dG-dC).poly(dG-dC), poly(dA-dT).poly(dA-dT), Br-p...

In this study, the influence of the functional groups by the diblock copolymers of poly(styrene-b-4-vinylpyridine) (PS-b-P4VP), poly(styrene-b-2-vinylpyridine) (PS-b-P2VP), and poly(styrene-b-methyl methacrylate) (PS-b-PMMA) on their blends with octa-functionalized phenol polyhedral oligomeric silsesquioxane (OP-POSS) nanoparticles (NPs) was investigated. The relative hydrogen bonding strengths...

Earlier, we showed that, for the D form (n = 8 and h = 3.03 A, where n is number of nucleotide units per turn and h is height per nucleotide unit) of poly[d(A-T)], both right- and left-handed double helical models are stereochemically satisfactory and give good agreement with the observed fiber diffraction data. It was also noted that the conformations of the right- and left-handed D-DNA models...

Abstract Background Combination chemo-photothermal therapy appears to be one of the next generations cancer treatment. In this study hollow gold nanostars (HGNSs) and nanocages (GNCs) were synthesized stabilized with thermo-pH-sensitive thiol-end capped ABC triblock copolymer poly(acrylic acid)-b-poly( N isopropylacrylamide)-b-poly (e-caprolactone)-SH; PAA-b-PNIPAAm-b-PCL-SH (GNSs@Pol). Doxorub...

Crystallization-driven self-assembly (CDSA) was employed for the preparation of monodisperse cationic cylindrical nanoparticles with controllable sizes, which were subsequently explored their effect on antibacterial activity and mechanical properties nanocomposite hydrogels. Poly(ɛ-caprolactone)-block-poly(methyl methacrylate)-block-poly[2-(tert-butylamino) ethyl methacrylate] (PCL-b-PMMA-b-PTA...

Proton 2D-NOE spectroscopy has been used to investigate the three-dimensional conformations of several sonicated polydeoxynucleotides in solution. The observed pattern of cross peaks indicate that poly(dAdT) . poly(dAdT1 in all salt concentrations studied (up to 6.6M CsF), and poly(dG-m5dC) . poly(dG-m5dC) in low salt (0.1M NaCU are righthanded B-structures. Poly(dG-m5dC) . poly(dG-m5dC) in Mg2...

The effect of block sequence on the self-assembly of ABC terpolymers in selective solvent (water/THF) was explored based on two kinds of terpolymers: polystyrene-block-poly(ethylene oxide)-block-poly(acrylic acid) (PS-b-PEO-b-PAA) and PEO-b-PS-b-PAA with the same component, comparable molecular weight, but different block sequence. Copolymer PS-b-PEO-b-PAA has a higher critical water content (C...

A general and simple method was presented for preparing supramolecular hydrogels to deliver anticancer drugs. In this system, hydrophobic anticancer drug doxorubicin (Dox) was loaded into poly(ethylene glycol)-b-poly(ε-caprolactone) (PEG-b-PCL) amphiphilic block copolymer micelles by hydrophobic interaction. The drug loaded micelles were then mixed with α-cyclodextrin (α-CD) solution to generat...

For a binary integer program (IP) \(\max c^\mathsf {T}x, Ax \le b, x \in \{0,1\}^n\), where \(A \mathbb {R}^{m \times n}\) and \(c {R}^n\) have independent Gaussian entries the right-hand side \(b {R}^m\) satisfies that its negative coordinates \(\ell _2\) norm at most n/10, we prove gap between value of linear programming relaxation IP is upper bounded by \(\text {poly}(m)(\log n)^2 / n\) with...