the genetic diversity of 89 flue-cured tobacco varieties was examined using 12 issr primers. these cultivarswere evaluated at the guilan tobacco research center, rasht, iran, using a 7×7 simple lattice design with two replications, and 12 morphological traits. the total number of pcr amplified products was 143 bands ranging from 450 to 3000 bp, of which 108 bands (74.28%) were polymorphic. prim...

to evaluate the genetic diversity of native populations of crown imperial (fritillaria imperialis l.) in zagross region, issr molecular along with 32 morphological traits were employed. a number of 160 samples corresponding to eight zagross populations distributed in six provinces were collected and assessed. using six primers, 57 bands were totally produced from which 49 were polymorphic. mean...

DNA methylation as epigenetic mark plays a key role in normal differential and developmental processes as well as in dynamic gene regulation at the genomic level. To assess DNA methylation pattern in different developmental stages of Aeluropus littoralis, methylation sensitive amplified polymorphism (MSAP) was used. Methylation and demethylation status at the CCGG recognition site were...

introduction despite the fact that iran is one of the major chickpea (cicer arietinum l.) center of origins, limited information is available regarding chickpea genetic variation and diversity. genetic diversity information is crucial for the choice of proper parents to establish new breeding programs. chickpea germplasm is poor, suggesting the need for gaining enough knowledge of genetic diver...

CURRENT SCIENCE, VOL. 81, NO. 3, 10 AUGUST 2001 248 by repeating the experiment at least once. A total of 34 decamer primers (series OPA, OPB etc.) from Operon Tech, CA, USA were screened using three representative genomic DNA samples of black pepper. Of these, 24 primers that yielded consistent and clear band patterns were selected for the final analysis of the 22 accessions used in the presen...

Random amplified polymorphic DNA fingerprinting was performed to assess the genetic diversity among rarely cultivated traditional indica rice (Oryza sativa L.) varieties collected from a tribal hamlet of Kerala State, India. A total of 664 DNA bands amplified by 15 primers exhibited 72.9% polymorphism (an average of 32.3 polymorphic bands per primer). The varieties Jeerakasala and Kalladiyaran ...

The accurate identification of orchardgrass (Dactylis glomerata L.) cultivars is necessary to ensure purity for consumers, the effective utilization of cultivars, and to protect the intellectual property for breeders. Therefore, this study aimed to use SSR to construct DNA fingerprinting of orchardgrass cultivars. The genetic diversity of 32 orchardgrass cultivars originated from 21 countries, ...

Random Amplified Polymorphic DNA (RAPD) markers were used to measure genetic diversity of Changium smyrnioides Wolff (Apiaceae), a genus endemic to eastern China and an endangered medicinal plant, collected from five populations along the Yangzi River. A total of 92 amplified bands were scored from the 13 RAPD primers, and a mean of 7.1 amplified bands per primer and 69% (64 bands) percentages ...

Retrotransposons are abundant in the genomes of plants. In the present study, inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) markers were developed for the cassava genome (Manihot esculenta Crantz). Four cassava cultivars (Fécula Branca, IPR-União, Olho Junto, and Tamboara, two samples per cultivar) were used to obtain IRAP ...

Erianthus arundinaceus is not only a candidate plant for sugarcane breeding programs, but also a potential bioenergy grass. Genetic variation that is affected by geographic environments and ploidy level is very important for the utilization of Erianthus arundinaceus. In this study, effects of geographic environments and ploidy level on genetic variation were studied through analyzing the geneti...