In vitro fermentabilities of two mushrooms (Lentinus edodes--LenS; Tremella fuciformis--TreS), an herb (Astragalus membranaceus--AstS), and their polysaccharide fractions (LenE, TreE, and AstE) were investigated using microflora from chicken ceca. Polysaccharides were extracted using the hot water method. The mushrooms had lower polysaccharide yields (8 to 10%) than the herb (31%). Fermentation...

The colloidal organic matter (COM) was isolated from the exudates of three cultured phytoplonkters, namely chlophyte nanoflagellate Tetraselmis sp., diatom Chaetoceros socialis and dinoflagellate Prorocentrum minimum, Gulf Trieste (northern Adriatic Sea). isolation COM performed by ultrafiltration with molecular weight cut-off membranes 5 kDa final desalinisation dialysis. composition character...

Studies have been made by means of sedimentation in the ultracentrifuge, and by diffusion and electrophoresis, to determine the molecular weights and homogeneity of the tuberculin protein and polysaccharide molecules as found in their natural state in the unchanged filtrates from culture media after growth of tubercle bacilli. These results have been compared with data obtained on fractions iso...

BACKGROUND Lipopolysaccharide derived from Pantoea agglomerans (LPSp) mainly consists of two aggregates, the high-molecular aggregate (HMM-LPSp) and the low-molecular aggregate (LMM-LPSp). The structural differences between HMM-LPSp and LMM-LPSp seem to depend on the length of the O-antigen polysaccharide because the lipid A regions of the two fractions are quite similar. In this study, we exam...

By the methods most generally used in the past for the purification of the tuberculin protein nucleic acid and polysaccharide often have not been removed. While in most of the final fractions the nucleic acid content varied from 4 to 8 per cent, in a few cases it was as high as 30 to 40 per cent and in others as low as 0.8 per cent. Such a low figure as the latter, however, could not bc attaine...

A hot water extract of Sasa veitchii is a health-promoting food in general use. To analyze the structure and function of the polysaccharide fraction of the extract, a macromolecular fraction was obtained by dialysis (HMF) and two polysaccharide fractions (NPS and APS) were separated by DEAE-Sephadex chromatography. HMF strongly reacted with human sera and immunoglobulin preparations. All of the...

We have examined the antibody response to Helicobacter pylori lipopolysaccharide (LPS) during natural infection in humans. The sera of over 70% of H. pylori-infected individuals were found to contain immunoglobulin G antibodies against the LPS fractions isolated from smooth strains of H. pylori but not against those derived from rough strains, as determined by enzyme-linked immunosorbent assay....

St. John's Wort herb extracts often contain undesirable or volitional polysaccharides. As polysaccharides exhibit structure-dependent biological functions in the present study water-soluble polysaccharides were extracted from herb material, fractionated by anion exchange chromatography into four main polysaccharide fractions (denominated as Hp1, Hp2, Hp3 and Hp4) and characterized by HPAEC-PAD,...

Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator. However, the components of TPPPS have not been recognized. To investigate the composition of TPPPS, crude polysaccharide was obtained from Taishan P. massoniana pollen through water extraction and ethanol precipitation. Three homogeneous poly...

An antigen common to Enterobacteriaceae and closely associated with endotoxin fractions has been separated by chromatography on DEAE cellulose employing elution with a NaCl gradient. The purified common antigen fails to coat erythrocytes, is poorly, if at all antigenic, it is non-dialyzable and excluded from sephadex G-100 gel. It is composed of polysaccharide and polypeptide. The most importan...