Studies have indicated that specific heme delivery to apocytochrome c is a critical feature of the cytochrome c biogenesis pathways called system I and II. To determine directly the heme requirements of each system, including whether other metal porphyrins can be incorporated into cytochromes c, we engineered Escherichia coli so that the natural system I (ccmABCDEFGH) was deleted and exogenous ...

Due to their potential applications for optical communications, data storage, and electrooptical signal processing, molecules with nonlinear optical (NLO) properties have been extensively investigated.’ As part of our interest in porphyrins and metalloporphyrins as photoresponsive2 and field-responsive material^,^ we have synthesized a series of “push-pull” porphyrins containing both donor and ...

Photodynamic inactivation (PDI) is an efficient approach for the elimination of a series of microorganisms; however, PDI involving phytopathogenic filamentous fungi is scarce in the literature. In the present study, we have demonstrated the photoinactivating properties of five cationic meso-(1-methyl-4-pyridinio)porphyrins on conidia of the phytopathogen Colletotrichum graminicola. For this pur...

Improved photosensitizers for use in photomedicine must possess good water-solubility and optimal photophysical properties. Phosphorus(V) porphyrins fulfill these criteria and are a class of porphyrins with significant potential applications in phototherapy. Five phosphorus(V) porphyrins bearing alkyl substituents have been synthesized. Reasonable to good yields were obtained for all P(V) inser...

In the preceding paper (1) the isolation and culture of Chlorella WEB-17 was described. Evidence was presented that this mutant produced three dicarboxylic porphyrins in relatively large amounts. In this paper we report the isolation from the porphyrin mixture of one of these dicarboxylic porphyrins and its characterization as hematoporphyrin isomer IX (Fig. 1). Hematoporphyrin has not previous...

It has been widely reported that the Harderian gland, present in most vertebrates, accumulates high levels of porphyrins, particularly protoporphyrin. The present study describes the extraction, identification and characterization of a group of hitherto unreported protoporphyrin glycoconjugates in the rat Harderian gland using HPLC, capillary electrophoresis, on-line HPLC/electrospray ionizatio...

The reactions of manganese(III) porphyrin complexes with terminal oxidants, such as m-chloroperbenzoic acid, iodosylarenes, and H(2)O(2), produced high-valent manganese(V)-oxo porphyrins in the presence of base in organic solvents at room temperature. The manganese(V)-oxo porphyrins have been characterized with various spectroscopic techniques, including UV-vis, EPR, 1H and 19F NMR, resonance R...

Four Zn(II)-tetra(carboxyphenyl)porphyrins in solution and bound to metal oxide (TiO2, ZnO, and ZrO2) nanoparticle films were studied to determine the effect of the spacer length and anchoring group position (para or meta) on their binding geometry and photoelectrochemical and photophysical properties. The properties of three types of anchoring groups (COOH and COONHEt3) for four Zn(II)-porphyr...

Open porous and transparent microcolumnar structures of TiO₂ prepared by physical vapour deposition in glancing angle configuration (GLAD-PVD) have been used as host matrices for two different fluorescent cationic porphyrins, 5-(N-methyl 4-pyridyl)-10,15,20-triphenyl porphine chloride (MMPyP) and meso-tetra (N-methyl 4-pyridyl) porphine tetrachloride (TMPyP). The porphyrins have been anchored b...

The classical procedure for the identification of hemins as prosthetic groups is to isolate the hemins, convert them into porphyrin esters, and identify the esters by their spectra and melting points. This procedure usually requires considerable amounts of material, gives poor yields, and the melting points have not always been definitive (1). Chromatographic methods have aided in establishing ...