Potato virus Y is the most economically important potato viral pathogen. We aimed at unraveling the roles of small RNAs (sRNAs) in the complex immune signaling network controlling the establishment of tolerant response of potato cv. Désirée to the virus. We constructed a sRNA regulatory network connecting sRNAs and their targets to link sRNA level responses to physiological processes. We discov...

Spetz, C. 2003. Molecular studies on a complex of potyviruses infecting solanaceous crops, and some specific virus-host interactions. Doctor’s dissertation ISSN 1401-6249, ISBN 91-576-6452-8 This thesis constitutes a comprehensive analysis of the molecular and biological characteristics of three potyviruses (genus Potyvirus, family Potyviridae) naturally occurring in cultivated and wild species...

Plant microRNAs (miRNAs) play pivotal roles in many biological processes. Although many miRNAs have been identified in various plant species, the functions of these miRNAs remain largely unknown due to the shortage of effective genetic tools to block their functional activity. Recently, miRNA target mimic (TM) technologies have been applied to perturb the activity of specific endogenous miRNA o...

18 Potato virus Y is the most economically important potato viral pathogen. We aimed at 19 unraveling the roles of small RNAs (sRNAs) in the complex immune signaling network 20 controlling the establishment of tolerant response of potato cv. Désirée to the virus. We 21 constructed a sRNA regulatory network connecting sRNAs and their targets to link sRNA 22 level responses to physiological proce...

In this study, the recovery phenomenon following infection with Potato virus Y (PVY) was investigated in tobacco (Nicotiana tobaccum), tomato (Solanum lycopersicum) and potato (Solanum tuberosum) plants. In tobacco plants, infection of severe strains of PVY (PVYN or PVYN:O) induced conspicuous vein clearing and leaf deformation in the first three leaves above the inoculated leaves, but much mil...

alfalfa mosaic virus (almv) is one of the most conspicuous alfalfa viruses in the world. in order to identify the distribution, host range and molecular characterization of alfalfa mosaic virus in kerman province, 514 alfalfa samples showing mosaic, calico, mottle and malformation symptoms as well as 66 potato samples showing calico symptoms were collected from alfalfa and potato fields in kerm...

One of the functions of RNA silencing in plants is antiviral defense. A hallmark of RNA silencing is spreading of the silenced state through the plant. Little is known about the nature of the systemic silencing signal and the proteins required for its production, transport, and reception in plant tissues. Here, we show that the RNA-dependent RNA polymerase RDR6 in Nicotiana benthamiana is invol...

The p24 protein, one of the three proteins implicated in local movement of potato virus X (PVX), was expressed in transgenic tobacco plants (Nicotiana tabacum Xanthi D8 NN). Plants with the highest level of p24 accumulation exhibited a stunted and slightly chlorotic phenotype. These transgenic plants facilitate the cell-to-cell movement of a mutant of PVX that contained a frameshift mutation in...