Hypersensitive response (HR)-conferred resistance to viral infection restricts the virus spread and is accompanied by the induction of cell death, manifested as the formation of necrotic lesions. While it is known that salicylic acid is the key component in the orchestration of the events restricting viral spread in HR, the exact function of the cell death in resistance is still unknown. We sho...

A severe disease of tomato was observed in 2010 in a greenhouse in the province of Lecce (Apulia, southern Italy). Plants showed interveinal yellowing and thickening of mature leaves and a bushy appearance of the new growth. Pale-yellow spots, which became sunken and necrotic, were scattered on the fruit surface. Leaf symptoms were reminiscent of those induced by Tomato chlorosis virus (ToCV) o...

Potato virus Y (PVY) is the type member of the potyvirus group that is the largest of the plant virus groups. Among several PVY strains, PVY° strains are spread worldwide (1) and PVY-O is one of Japanese PVY° strains. We propagated PVY-0 on Nicotiana tabacum cv. Samsun NN and isolated its genomic RNA. The synthesized double-stranded cDNA was inserted into the EcoKl site of pBR322 using £coRI li...

Potato virus Y (PVY) impacts potato production worldwide by reducing yield, tuber quality, and successful certification of seed. Extreme resistance to PVY is conferred three genes (Ryadg, Rysto, Rychc) which have been introgressed from wild species into cultivated varieties. Both Rysto Ryadg are used in many breeding programs worldwide, but Rychc found only a limited number varieties previously...

BACKGROUND Potato virus Y (PVY, genus Potyvirus) causes substantial economic losses in solanaceous plants. Routine screening for PVY is an essential part of seed potato certification, and serological assays are often used. The commercial, commonly used monoclonal antibodies, MAb1128, MAb1129, and MAb1130, recognize the viral coat protein (CP) of PVY and distinguish PVYN strains from PVYO and PV...

Aim. Screening of vegetable crops for mixed viral infections caused by 8 viruses, and evaluation relative share different combinations pathogens using serological methods plant virus diagnostics. Methods. Double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was performed detection antigens. Results. For this study, the samples from plants with virus-like symptoms were collecte...

Two serologically unrelated sweet potato viruses causing symptoms of vein clearing in the indicator plant Ipomoea setosa were isolated and their genomes have been sequenced. They are associated with symptomless infections in sweet potato but distinct vein-clearing symptoms and higher virus titres were observed when these viruses co-infected with sweet potato chlorotic stunt virus (SPCSV), a vir...

Mitogen-activated protein kinase (MAPK) cascades have crucial roles in the regulation of plant development and in plant responses to stress. Plant recognition of pathogen-associated molecular patterns or pathogen-derived effector proteins has been shown to trigger activation of several MAPKs. This then controls defence responses, including synthesis and/or signalling of defence hormones and act...