A simple, rapid, inexpensive method for the localization of virus transcripts in plant and insect vector tissues is reported here. The method based on fluorescent in situ hybridization using short DNA oligonucleotides complementary to an RNA segment representing a virus transcript in the infected plant or insect vector. The DNA probe harbors a fluorescent molecule at its 5' or 3' ends. The prot...

greenhouse whitefly and two-spotted spider mite are two important pests on greenhouse crops. the polyphagous predatory mite amblyseius swirskii preys on both of these pests. in this research, predation of the predatory mite on different stages of both pests, prey stage preference of pests, prey preference and switching behavior of predator between preferred stage of greenhouse whitefly and two-...

background: gossypium arboreum is resistant to cotton leaf curl burewala virus and its cognate cotton leaf curl multan betasatellite (clcubuv and clcumb). however, the g. arboreum wax deficient mutant (gawm3) is susceptible to clcuv. therefore, epicuticular wax was characterized both quantitatively and qualitatively for its role as physical barrier against whitefly mediated viral transmission a...

Three new species of Baeoentedon Girault (Hymenoptera: Eulophidae) are described from China, Baeoentedon balios Wang, Huang & Polaszek sp. nov., Baeoentedon bouceki Wang, Huang & Polaszek sp. nov. and Baeoentedon virgatus Wang, Huang & Polaszek sp. nov. Both female and male of Baeoentedon balios were reared from the whitefly Pealius spina (Singh) (Hemiptera: Aleyrodidae) on the bodhi tree Ficus...

Facultative bacterial endosymbionts are common, influential associates of arthropods, yet their movement among host species has not been well documented. Plant-mediated transmission of Rickettsia has been shown for the whitefly Bemisia tabaci. Bemisia tabaci in USA cotton fields harbors the secondary symbionts Rickettsia and Hamiltonella, and co-occurs with Trialeurodes sp. nr. abutiloneus whit...

An accurate and simple evaluation method is crucial for identifying whitefly resistance in tomato breeding. We developed an in vitro method for evaluating resistance of tomato leaves and tested this on wild and cultivated tomato varieties. We found that young leaves observed for whitefly oviposition after 8 hours provided appropriate comparative conditions. This method effectively distinguished...