A one-step fluorescence polarization immunoassay (FPIA) was developed to measure progesterone level using an immunocomplex single reagent (SR), a preequilibrated mixture of antibody and tracer. Several fluorescence-labeled progesterone tracers were synthesized using the combination of two progesterone derivatives, 11alpha-hemisuccinyloxyprogesterone (P-11HS) and progesterone-3-(O-carboxymethyl)...

the aim of the study was to investigate the effect of tail-docking on the lh pulse frequency in femaletuj lambs. twelve female lambs were assigned into two equal groups. a rubber ring was applied to the baseof the tail for shedding off. blood samples were collected for 8 h in the 4th and 8th months of age todetermine pulsatile secretion of lh. starting from the age of 6 months, blood samples we...

Progesterone causes a rapid increase in the intracellular free calcium level in fully grown amphibian oocytes. When albino Xenopus laevis oocytes were microinjected with the Ca-specific photoprotein aequorin, the calcium-induced luminescence from this protein increased at 40-60 sec after the addition of progesterone and returned to the control level within 5-6 min. No further change in the aequ...

Plasma levels of progesterone and corticoids were determined in eight sows prior to, during and following parturition. A statistically significant diurnal variation was observed with morning samples having a higher level of corticoids. Progesterone levels decreased through the last day of gestation to a low level at day 1 postpartum. Mean corticoid levels increased during the last 24-hr. prepar...

Xenopus oocytes mature in response to the steroid hormone progesterone. At the level of a population of oocytes, the response is graded-the higher the concentration of progesterone, the larger the fraction of oocytes that will mature-but at the level of the individual oocyte, the response is all-or-none. The all-or-none character of this cell fate switch is hypothesized to arise out of two prop...

OBJECTIVE To assess the relationship between the estrogen-progesterone alterations before and after ovulation trigger and treatment success in intrauterine insemination (IUI) cycles. MATERIALS AND METHODS Two hundred fifty-one women with infertility underwent ovulation induction followed by IUI. For all subjects, estradiol and progesterone concentrations were evaluated on the trigger and IUI ...

The mechanisms of estrogen and progesterone in human cutaneous pigmentation are largely unknown. The molecular identification of estrogen receptor (ER) and progesterone receptor (PR) in the human melanocytes is of great importance to understand the mechanisms. We performed immunocytochemistry analysis and demonstrated that ER and PR were expressed in the cytoplasms and nuclei of human melanocyt...