Excess reactive oxygen species (ROS) beyond the scavenging capacity of antioxidants leads to DNA damage and oxidation of lipoprotein components at the cellular and subcellular level. The oxidative stress (OS) adversely affects sperm function by altering membrane fluidity, permeability and impairs sperm functional competence. In the present study, the OS status in seminal plasma and blood serum ...

BACKGROUND Application of follicular fluid (FF) and platelet-activating factor (PAF) in artificial insemination improves sperm motility. Lactate dehydrogenase C (LDH-C) is a key enzyme for sperm motility. In this study, the effects of FF and PAF on the sperm motility index and LDH-C expression were investigated. Moreover, LDH-C expression was compared between asthenozoospermic and normozoosperm...

AIM The aim of this study was to investigate the effect of incubation on freezability of cholesterol loaded cyclodextrin (CLC) treated buffalo spermatozoa. MATERIALS AND METHODS Semen samples with mass motility of 3+ and greater, collected from Murrah buffalo bulls were utilized. Immediately after collection, four equal groups of semen sample were made. Group I was kept as control and diluted...

Context. This study follows a recent analysis of the galaxy luminosity functions and colour-magnitude red sequences in the Coma cluster (Adami et al. 2007). Aims. We analyze here the distribution of very faint galaxies and globular clusters in an east-west strip of ∼ 42 × 7 arcmin 2 crossing the Coma cluster center (hereafter the CS strip) down to the unprecedented faint absolute magnitude of M...

four mature stallions were used to study the effect of enzymatic antioxidant catalase on freezability of turkmen stallion's sperm. collected semen from stallions were processed and pooled before being divided treatments (200×106  sperm/ml) and diluted with extenders supplemented with different levels of catalase [0 (control), 100 u/ml (cat100), 150u/ml (cat150) and 200u/ml (cat200)]. exten...

antioxidant effect of olive leaf extract (ole) was studied on motility, viability, plasma membrane integrity of spermatozoa and malondialdehyde production in 12 ross 308 roosters at their 30 weeks of age. semen samples were collected by abdominal massage in 5 times. in each session after the initial sperm assessment, collected samples were pooled and diluted with sexton extender.samples were sp...

Antioxidant properties of ghrelin have been demonstrated in recent studies. In the present study, theeffects of chronic administration of ghrelin on the motility and plasma membrane integrity of rat spermatozoa during incubation at 37ºC were investigated. Thirty 45-day-old male Wistar rats were divided into control and treatment groups. Rats in the treatment group were daily injected subcutaneo...

objective: the aim of the present study was to investigate the effects of four equilibration times (2, 4, 8 and 16 hours) and two extenders (tris or bioxcell®) on cryopreservation of buffalo semen. materials and methods: in this experimental study, split pooled ejaculates (n=4), possessing more than 70% visual sperm motility were divided in two aliquots and diluted in bioxcell ® and tris-citric...

The time required to train rams to an artificial vagina (AV) makes collecting semen from large numbers of rams difficult. To manage this problem, we developed a glass, round-bottomed, 1.9-cm i.d. x 9.8-cm long vaginal collection vial (VCV). Three experiments were conducted to determine whether the VCV affected 1) semen volume per collection, 2) percentage of motile spermatozoa, 3) forward progr...