Antisense oligonucleotides targeting microRNAs or their mRNA targets prove to be powerful tools for molecular biology research and may eventually emerge as new therapeutic agents. Synthetic oligonucleotides are often contaminated with highly homologous failure sequences. Synthesis of a certain oligonucleotide is difficult to scale up because it requires expensive equipment, hazardous chemicals ...

Japanese pear (Pyrus pyrifolia) exhibits gametophytic self-incompatibility (GSI) controlled by a complex and multiallelic S locus. The pistil-part product of the S locus is the polymorphic ribonuclease, S-RNase. Information on S-genotypes is important for the production and breeding of Japanese pears. Molecular analyses of S-genotypes of Japanese pear have been conducted with the CAPS (cleaved ...

The relative performance of a clear delta trap baited with individual or combination sex pheromone and kairomone lures for codling moth, Cydia pomonella L., was evaluated against three vertical clear pane and colored delta traps in field trials within apple, Malus domestica (Borkhausen). The clear caught significantly more moths of each sex than an orange delta trap when baited with ethyl (E,Z)...

to study the tolerance of genotypes under terminal drought stress, 20 promising winter and facultative bread wheat genotypes were evaluated at karadj, ardebil, arak and hamedan agricultural research stations during 2001-02 cropping season. the experimental design in all the locations was randomized complete block of three replications. grain yield (yld), harvest index (hi), grain number per spi...

Abstract Studies were conducted in apple, Malus domestica Borkhausen and pear, Pyrus communis L. (Rosales: Rosaceae), orchards to evaluate the attractiveness of grey halobutyl septa loaded with 1 (L2) and 10 (Mega) mg of codlemone, 8E, 10E-dodecadien-1-ol, 3 mg of pear ester, ethyl (E,Z)-2,4-decadienoate (DA2313), and 3 mg of pear ester plus 3 mg of codlemone (Combo) to adult codling moth, Cydi...

The enterobacterium Erwinia amylovora is a devastating plant pathogen causing necrotrophic fire blight disease of apple, pear, and other rosaceous plants. In this study, we used a modified in vivo expression technology system to identify E. amylovora genes that are activated during infection of immature pear tissue, a process that requires the major pathogenicity factors of this organism. We id...

Metabolites in the fruits of edible plants include sweet sugars, visually appealing pigments, various products with human nutritional value, and biologically active plant hormones. Although quantities of these metabolites vary during fruit development and ripening because of cell division and enlargement, there are few reports describing the actual dynamics of these changes. Therefore, we appli...

Pear (Pyrus spp) is an important fruit crop, grown in all temperate regions of the world, with global production ranked after grape and apples among deciduous tree crops. A high-density linkage map is a valuable tool for fine mapping quantitative trait loci (QTL) and map-based gene cloning. In this study, we firstly constructed a high-density linkage map of pear using SNPs integrated with SSRs,...

conventional methods of pear breeding, largely based on intra- and inter-specific hybridization, are difficult because pear is highly heterozygous, polygenic and has a long juvenile period. genetic improvements of pear cultivars are possible through induction of mutations and gene transfer by genetic engineering. a general prerequisite for these approaches is to establish an efficient plant reg...