Single-particle electron cryo-microscopy (cryo-EM) is an emerging tool for resolving structures of conformationally heterogeneous particles; however, each structure is derived from an average of many particles with presumed identical conformations. We used a 3.5-Å cryo-EM reconstruction with imposed D7 symmetry to further analyze structural heterogeneity among chemically identical subunits in e...

Small heat shock proteins (sHSPs) are ubiquitous chaperones that bind and sequester non-native proteins preventing their aggregation. Despite extensive studies of sHSPs chaperone activity, the location of the bound substrate within the sHSP oligomer has not been determined. In this paper, we used cryoelectron microscopy (cryoEM) to visualize destabilized mutants of T4 lysozyme (T4L) bound to en...

The oligomer of β-amyloid (Aβ) is considered the main neurotoxin in Alzheimer's disease (AD). Therefore, the inhibition of the formation of Aβ oligomer could be a target for AD therapy. In this study, with the help of the dot blotting assay and transmission electronic microscopy, it was have discovered that 5-hydroxycyclopenicillone, a cyclopentenone recently isolated from a sponge-associated f...

Photocatalytic oxidation of the sulfur-containing compounds, trimethylene sulfide (C3H6S), propylene sulfide (C3H6S), thiophene (C4H4S), and methyl disulfide (C2H6S2), was carried out using an annular plug flow reactor with TiO2 in a supported form. Formation of products and byproducts was monitored in real time using a mass spectrometry online system. Mineralization of the sulfur-containing co...

l'auteur explique le comportement des esters cycliques neutres vis -a- vis de l'eau ,de l'acide chlorhydrique , ainsi que la polymerisation des ethylphosphates cycliques d'ethylene et de propylene par des traces d'eau. les produits resultant de la plymerisation de ce dernicr phosphate cyclique sont plus complexes que ceux obtenus dans les memes conditions avec 1'et...

Many organic-rich rocks are major sources of oil and gas in sedimentary basins presumably through high-temperature thermal cracking. This view was brought into question with recent reports of marine shales generating catalytic gas in the laboratory at 50◦C, 300◦C below thermal-cracking temperatures. Gas forms under natural conditions without artificial stimulation. Compositions of methane, etha...

Carbon nanotubes feature excellent electronic properties but narrow absorption bands limit their utility in certain optoelectronic devices, including photovoltaic cells. Here, the addition of a wide-bandgap gap oligomer enhances light absorption in the visible spectrum. Furthermore, the oligomer interacts with the carbon nanotube through a peculiar charge transfer, which provides insight into T...

Recent in vitro and in vivo studies suggest that destabilized proteins with defective folding induce aggregation and toxicity in protein-misfolding diseases. One such unstable protein state is called amyloid oligomer, a precursor of fully aggregated forms of amyloid. Detection of various amyloid oligomers with A11, an anti-amyloid oligomer conformation-specific antibody, revealed that the amylo...

Propylene glycol (PG) is a solvent commonly used in medications that, while benign at low doses, may cause toxicity in adults and children at high doses. We describe a case and the physiologic sequelae of propylene glycol toxicity manifested in a critically ill adolescent male with refractory myoclonic status epilepticus aggressively treated with multiple PG-containing medications (lorazepam, p...

Vesicles formed in water by synthetic macro-amphiphiles have attracted much attention as nanocontainers having properties that extend the physical and chemical limits of liposomes. We sought to develop ABA block copolymeric amphiphiles that self-assemble into unilamellar vesicles that can be further oxidatively destabilized. We selected poly(ethylene glycol) (PEG) as the hydrophilic A blocks, o...