Objective: The study aims to perform molecular docking examine the interaction between propolis compound and SARS-CoV-2 main protease.
 Methods: protein target of this research was crystal structure protease in complex with an inhibitor N3 (PDB ID: 6LU7). ligand bioactive compounds from Propolis Tetragonula aff. biroi. 
 Results: results showed that which has potential inhibit activit...

In addition to NS3 protease, the NS4A protein is required for efficient cleavage of the nonstructural protein region of the hepatitis C virus polyprotein. To investigate the function and the sequence of NS4A required for the enhancement of NS3 protease activity, we developed an in vitro NS3 protease assay system consisting of three purified viral elements: (i) a recombinant NS3 protease which w...

A sequential optimization strategy, based on statistical experimental designs, is employed to enhance the production of alkaline protease by a Bacillus pseudofirmus local isolate. To screen the bioprocess parameters significantly influencing the alkaline protease activity, a 2-level Plackett-Burman design was applied. Among 15 variables tested, the pH, peptone, and incubation time were selected...

Care of chronic and stalled wounds is hampered by the lack of diagnostic tools to help direct clinicians to specific treatments or diagnose specific conditions. Studies have shown a correlation between high protease levels and nonhealing wounds; a diagnostic protease test is under development. Seven wound care experts (two podiatrists, two vascular surgeons, a physician expert in hype...

Rothia mucilaginosa is a part of the normal oral microflora, which has been recognized as an endodontic pathogen and also emerging opportunistic in immune compromised hosts. In this study, we identified rhomboid protease R. candidate virulence factor. A database search two genes on genome with open reading frames 828 bp 822 bp, respectively. The are separated by 57 suggesting that they constitu...

We have developed a high throughput assay for the measurement of protease activity in solution. This technology will accelerate research in functional proteomics and enable biologists to streamline protease substrate evaluation and optimization. The peptide sequences that serve as protease substrates in this assay are labeled on the carboxy terminus with a biotin moiety and a fluorescent tag is...