We have developed a coevolutionary method for the computational design of HIV-1 protease inhibitors selected for their ability to retain efficacy in the face of protease mutation. For HIV-1 protease, typical drug design techniques are shown to be ineffective for the design of resistance-evading inhibitors: An inhibitor that is a direct analogue of one of the natural substrates will be susceptib...

Sequence logos are frequently used to illustrate substrate preferences and specificity of proteases. Here, we employed the compiled substrates of the MEROPS database to introduce a novel metric for comparison of protease substrate preferences. The constructed similarity matrix of 62 proteases can be used to intuitively visualize similarities in protease substrate readout via principal component...

This research focused on the isolation and characterization of new strains which can be used as microbial resources for auxin production in agriculture industry. For microorganism gut Larimichthys polyactis, a marine agar medium was 3 colonies were isolated. Through 16S-based ID service, isolated identified 1 strain Niallia circulans Proteus mirabilis. Verifying industrial values these strains,...

Microbes attach in pickle leather, wet blue and upper shoeleather have been identified to positive profile produce extracellular protease enzymes with pH values of acidic basic on 15% gelatin 2% commercial skimmed milk as substrates. Defective tanned leather was added a microbial growth solution enriched substrate incubated for 7 days at room temperature an open rotary shaker 120 rpm then store...

Edible fungi are often used as an important selenium (Se) source to improve human daily Se intakes food or supplements because of their strong ability accumulate Se, in which Ganoderma lucidum was widely accepted China. However, the existing speciation analysis methods such protease K-based trypsin-protease XIV-based, have low extraction rate and enzymatic hydrolysis (<30%) on Se-enriched ed...

The reclusive enzymes that catalyze proteolysis inside the cell membrane are among the most widespread in nature, yet most remain ‘orphans’ whose cellular functions are poorly understood. Now, Voss et al identify Golgi-resident glycosyltransferases and glycosidases as substrates for the presenilin-like protease SPPL3. Shedding of these glycan-modifying enzymes from the membrane down-regulates g...

In this study, Enterococcus faecalis proteolytic strains which have the potential to degradation of bovine milk proteins were isolated from Turkish White Pickled Cheeses and samples. E. found strong caseinolytic activity. The extracellular protease enzymes produced by 60 different samples analyzed in pattern bands on a stained SDS-PAGE gel. highest activity isolates determined at pH 7.0 40 ℃ fo...

COVID-19 pandemic is the biggest public health problem of century so far.The main protease (Mpro) one enzymes studied as a pharmacological target. In this context, present work aimed to perform virtual screening possible inhibitors against enzyme Mpro, having limonoids object research supposed inhibitors. Molecular docking simulations indicated that have an affinity complex with M-pro.However, ...

The functional life of the flower is terminated by senescence and/or abscission. Multiple processes contribute to produce the visible signs of petal wilting and inrolling that typify senescence, but one of the most important is that of protein degradation and remobilization. This is mediated in many species through protein ubiquitination and the action of specific protease enzymes. This paper r...

The PST-01 protease is highly stable and catalyzes the synthesis of the aspartame precursor with high reaction yields in the presence of organic solvents. However, the synthesis rate using the PST-01 protease was slower than that observed when thermolysin was used. Structural comparison of both enzymes showed particular amino acid differences near the active center. These few residue difference...