The nucleotide sequences of the matrix protein (M) genes of two wild-type measles virus (MV) isolates (JM and CM) have been determined and shown to differ in 56 positions; 31 of these differences are located in the non-coding region and 25 in the coding region of the gene. Most (80%) of the mutations in the coding region are changes to the third base of a codon. A maximum parsimony analysis of ...

A 2.3-kb BamHI-KpnI fragment was isolated from a partial genomic library and shown by nucleotide sequence analysis to contain the entire coding region of the gene encoding the b subunit of the Blastocladiella mitochondrial processing peptidase (b-MPP). The predicted b-MPP protein has 465 amino acids and a calculated molecular mass of 50.8 kDa. S1 nuclease protection assays revealed an intron, 2...

results primary, secondary, and 3d structure of cchfv gn were obtained and pcr reaction with m13 forward and reverse primers confirmed the generation of recombinant bacmid dna harboring gn coding region under polyhedron promoter. conclusions characterization of the detailed structure of cchfv gn by bioinformatics software provides the basis for development of new experiments and construction of...

Reverse transcriptase of murine retroviruses is a monomeric protein of approximately 80,000 daltons, which is encoded by the central portion of the viral pol gene. To prepare large quantities of the enzyme, we have constructed gene fusions between the trpE gene and portions of the pol gene of Moloney murine leukemia virus. The inserted pol gene sequences include the entire coding region for the...

beet yellows virus belong to the genus closterovirus in the closteroviridae family which is one the most important viruses of sugar beet worldwide. in order to identify and characterize some molecular aspects of the byv, 48 symptomatic leaf samples of sugar beet were collected from sugar beet field in khorasan razavi province of iran. leaves showing general yellowing, vein clearing and thicknes...

A 2.3-kb BamHI-KpnI fragment was isolated from a partial genomic library and shown by nucleotide sequence analysis to contain the entire coding region of the gene encoding the beta subunit of the Blastocladiella mitochondrial processing peptidase (beta-MPP). The predicted beta-MPP protein has 465 amino acids and a calculated molecular mass of 50.8 kDa. S1 nuclease protection assays revealed an ...

The EIF4E gene spans >50 kbp and contains 8 exons, one of them being alternative. It codes for the major, 4749 nucleotides long transcript variant 1, that codes 217 aa long protein. The longest transcript, variant 2 (4842 nucleotides) contains an additional in-frame exon in 3' coding region compared to variant 1 and codes for a protein (248 aa long, isoform 2) with a longer cterminus compared t...

Recombinant protein translation in Escherichia coli may be limited by stable (i.e. low free energy) secondary structures in the mRNA translation initiation region. To circumvent this issue, we have set-up a computer tool called 'ExEnSo' (Expression Enhancer Software) that generates a random library of 8192 sequences, calculates the free energy of secondary structures of each sequence in the -70...

very little is known about lhr and fshr genes of domestic dromedary camels. the main objective of this study was to determine and analyze partial genomic regions of fshr and lhr genes in dromedary camels for the first time. to this end, a total of 50 dna samples belonging to dromedary camels raised in iran were sent for sequencing (25 samples of each gene). we compared the nucleotide sequences ...

dna barcoding based on a standardized region of 648 base pairs of mitochondrial dnasequences from cytochrome c oxidase 1 (cox1) is proposed for animal species identification.recent studies suggested that dna barcoding has been effective for identifying 94% of birdspecies. the proposed threshold of 10 times the average intraspecific variation could be used forthe identification and delimitation ...