OBJECTIVES Immunoglobulin paraproteins can interfere with multiple chemistry assays. We want to investigate the mechanisms of immunoglobulin interference. DESIGN AND METHODS Serum samples containing paraproteins from the index patient and eight additional patients were used to investigate the interference with the creatinine and total protein assays on the Beckman Coulter AU5400/2700 analyzer...

We present the first microfluidic method for extracting proteins from heterogeneous fluids by precipitation. The new method comprises an automated protocol for precipitation of proteins onto surfaces, rinsing the precipitates to remove impurities, and resolubilization in buffer for further analysis. The method is compatible with proteins representing a range of different physicochemical propert...

Since Panum (1) in 1851 found that protein separated from serum on dilution and the addition of acid, the most widely used means of defining the plasma proteins has been by describing their solubility behavior. Between 1859 and 1902 the work of Denis (2), Hammarsten (3), Burckhardt (4), Marcus (5), Kauder (6), Pick (7), and others established the definitions of these proteins as commonly used t...

Plants provide an advantageous expression platform for biopharmaceutical proteins because of their low pathogen burden and potential for inexpensive, large-scale production. However, the purification of target proteins can be challenging due to issues with extraction, the removal of host cell proteins (HCPs), and low expression levels. The heat treatment of crude extracts can reduce the quantit...

Many seaweed species have a high production potential and attract interest as future protein sources. A fiber ash content, however, demand extraction of the to improve its digestibility utilization in food or feed. This study explores three different approaches for from Ulva fenestrata order maximize yield. Soluble was recovered either by mechanical pressing homogenization osmotic shock biomass...

Extracellular vesicles (EV) are important for delivering biologically active substances to facilitate cell-to-cell communication. Milk-derived EV widely known because of their potential immune enhancement. However, procedures isolating milk-derived have not been fully established. To obtain pure and accurately reveal function, such must be The aim the present study was compare methods using com...

Phytochemical constituents, such as total phenol, allicin and pyrovic acid as well as antioxidant activity of thirteen ecotypes of Allium hirtifolium Boiss. from different regions of Iran were evaluated. Average contents of total phenol, allicin and pyrovic acid of bulbs extracts per g fresh weight were 0.647 mg gallic acid equivalent (GAE), 2.127 mg and 84.412 µmol, respectively and antioxidan...