Proteins are polymers of amino acids. These macromolecules are synthesized by intracellular machines called ribosomes. Although the experimental investigation of protein synthesis has been a traditional area of research in molecular cell biology, important quantitative models of protein synthesis have been reported in research journals devoted to statistical physics and related interdisciplinar...

Positive-strand and double-strand RNA viruses typically compartmentalize their replication machinery in infected cells. This is thought to shield viral RNA from detection by innate immune sensors and favor RNA synthesis. The picture for the non-segmented negative-strand (NNS) RNA viruses, however, is less clear. Working with vesicular stomatitis virus (VSV), a prototype of the NNS RNA viruses, ...

(a) After injection of labeled leucine into the eye of goldfish, radioactive protein rapidly accumulates in the contralateral optic tectum in the layer containing the synaptic endings of the optic fibers. This material reaches the tectum 6-12 hr after the isotope injection, a fact which indicates that the rate of transport is at least 40 mm per day. (b) This rapidly transported material has bee...

introduction: an effective vaccine against hcv infection is not available. the non-structural protein 3 (ns3) of the virus as an important immunogenic candidate has been utilized in various modules. nanostructured polymers have been recently used for efficient vaccine and drug delivery. the aim of the current study was the synthesis of rns3-g2 conjugate and preliminary evaluation of its immunog...

1. Puromycin dihydrochloride inhibited uptake of CYaminoisobutyric acid (AID), 1-aminocyclopentane-I-carboxylic acid, and a, y-diaminobutyric acid by intact diaphragm muscle from male rats weighing 60 to 90 g when the antibiotic was added to the system in vitro 120 and 180 min before the addition of the substrate. 2. In the absence of insulin, this inhibitory response was also produced by cyclo...

1. Puromycin dihydrochloride inhibited uptake of CYaminoisobutyric acid (AID), 1-aminocyclopentane-I-carboxylic acid, and a, y-diaminobutyric acid by intact diaphragm muscle from male rats weighing 60 to 90 g when the antibiotic was added to the system in vitro 120 and 180 min before the addition of the substrate. 2. In the absence of insulin, this inhibitory response was also produced by cyclo...

Elongation factor RbbA is required for ATP-dependent deacyl-tRNA release presumably after each peptide bond formation; however, there is no information about the cellular role. Proteomic analysis in Escherichia coli revealed that RbbA reciprocally co-purified with a conserved inner membrane protein of unknown function, YhjD. Both proteins are also physically associated with the 30S ribosome and...

BUG6 is a temperature-sensitive cell division mutant which forms filaments at the nonpermissive temperature. Synthesis of the maltose- and galactose-binding protein-dependent transport systems is also temperature sensitive in BUG6. Using operon and protein fusions of the maltose transport genes to lacZ, we observed that the temperature-sensitive control of the maltose transport system in BUG6 o...

background: prediction of interaction sites within the membrane protein complexes using the sequence data is of a great importance, because it would find applications in modification of molecules transport through membrane, signaling pathways and drug targets of many diseases. nevertheless, it has gained little attention from the protein structural bioinformatics community. methods: in this stu...

an analytical model for a coastal boundary current was used to investigate heat and salt budget of exchange flows in the persian gulf as a marginal sea. coastal boundary currents exchange heat and freshwater with the mosphere and the offshore waters. as heat and salinity fluxes caused by air-sea interaction and eddy activities, different temperature and salinity associated with boundary current...