background: communication is very important in human societies in connection with the exchange of ideas and information between the instructor and student. the current research aimed to study factors that influence the interaction between the instructor and student from the perspective of shushtar medical sciences students. methods: this descriptive - cross sectional study was conducted in ahva...

We report a method of photo-cross-linking proteins in mammalian cells, which is based on site-specific incorporation of a photoreactive amino acid, p-benzoyl-L-phenylalanine (pBpa), through the use of an expanded genetic code. To analyze the cell signaling interactions involving the adaptor protein Grb2, pBpa was incorporated in its Src homology 2 (SH2) domain. The human GRB2 gene with an amber...

SEE PAGE 551 G uanine nucleotide-binding proteins, or G-proteins, typically provide the major signal transducing step between agonist occupancy of a 7-transmembrane receptor and effector mechanisms. G-proteins consist of 3 subunits (a, b, and g); the b and g subunits are tightly bound and typically function as a “bg” subunit. Both the a and bg subunits serve signal transduction functions. G-pro...

Ongoing improvements in Computational Biology (CB) research have generated massive amounts of Protein-Protein Interactions (PPIs) data set. In this regards, the availability of PPI data for several organisms provoke the discovery of computational methods for measurements, analysis, modeling, comparisons, clustering and alignments of biological data networks. Nevertheless, fixed network comparis...

The ability to identify protein binding sites and to detect specific amino acid residues that contribute to the specificity and affinity of protein interactions has important implications for problems ranging from rational drug design to analysis of metabolic and signal transduction networks. Support vector machines (SVM) and related kernel methods offer an attractive approach to predicting pro...

CHEMICAL PROBES FOR PROTEIN N-TERMINAL METHYLTRANSFERASE. Rong Huang, Gang Zhang, Yunfei Mao, & Stacie Lynn Richardson, Department of Medicinal Chemistry, Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond VA 23219. The objective of this study is to develop chemical probes for N-terminal RCC1 methyltransferase (NRMT1). NRMT transfers methyl groups fr...

the binding data for interaction of a homologous series of n-alkyl sulfates with alkyl chainlengths from c8 to c12 with insulin were analyzed on basis of hill equation for two classes ofbinding sites .the intrinsic gibbs free energies were calculated and resolute on basis ofelectrostatic and hydrophobic contributions the estimation of these contributions reveals themajor role of electrostatic i...

abstractintroduction: cancer is caused by genetic abnormalities, such as mutation of ontogenesis or tumor suppressor genes which alter downstream signaling pathways and protein-protein interactions. comparison of protein interactions in cancerous and normal cells can be of help in mechanisms of disease diagnoses and treatments. methods: we constructed protein interaction networks of cancerous a...

background: clopidogrel is a prodrug that converts in the liver to an active thiol metabolite, which irreversibly inhibits the platelet p2y12 adenosine diphosphate receptor. it seems that methadone as cyp2c19 inhibitor affects ticlopidine activity in vivo. this study aimed to test the ability of methadone in changing ticlopidine pharmacokinetics. methods: we conducted a case–control study in 10...