Bacillus subtilis protoplast fusion allows the study of the genetic recombination of an entire procaryotic genome. Protoplasts from bacterial strains marked genetically by chromosomal mutations were fused using polyethylene glycol and the regenerated cells analyzed. Recombinants represent 19.3% of heterozygotic cells; they are haploids. Individual characterization of clones show a unique partic...

Bacterial protoplasts, released from confinement within the rigid wall of whole cells, imbibe water, swell, and burst unless their suspending medium contains high concentrations of solutes which have difficulty crossing the osmotic barrier in the protoplast surface membrane (C. Weibull, Exptl. Cell Res. 9:139, 1955; P. Mitchell and J. Moyle, J. Gen. Microbiol. 20:434, 1959). Hence, protoplast p...

The paper considers theoretical data on the mechanisms of formation reactive oxygen species (ROS) and transduction stress signals in plant cells. negative effects ROS with excessive generation are described. A method protoplast isolation a fluorescent microscopy were selected for experiments. Also, this work, comparative analysis production during hypoxia reaeration agricultural plants that dif...

for half a century, the limitations of obtaining cross-combinations in lilies because of the incompatibility and incongruity between different varieties have been known. somatic hybridization is one of the most powerful tools for achieving distant interspecific hybrids. for this purpose, protoplast preparation is a first and important step in efficient system for the regeneration of plants from...